Gating a single cell: a label-free and real-time measurement method for cellular progression

Oo, S. L., Ilyas, A.-M., Karthikeyan, V., Arava, C. M., Kong, E. Y., Yeung, C.-C., Chen, X., Yu, P. K.N. and Vellaisamy, R. A.L. (2020) Gating a single cell: a label-free and real-time measurement method for cellular progression. Analytical Chemistry, 92(2), pp. 1738-1745. (doi: 10.1021/acs.analchem.9b03136) (PMID:31904934)

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Abstract

There is an ever-growing need for more advanced methods to study the response of cancer cells to new therapies. To determine cancer cells’ response from a cell-mortality perspective to various cancer therapies, we report a label-free and real time method to monitor the in situ response of individual HeLa cells using a single cell gated transistor (SCGT). As a cell undergoes apoptotic cell death, it experiences changes in morphology and ion concentrations. This change is well in line with the threshold voltage of the SCGT, which has been verified by correlating the data with the cell morphologies by scanning electron microscopy and the ion-concentration analysis by inductively-coupled plasma mass spectrometry (ICPMS). This SCGT could replace patch clamps to study single cell activity via direct measurement in real time. Importantly, this SCGT can be used to study the electrical response of a single cell to stimuli that leaves the membrane intact.

Item Type:Articles
Additional Information:We acknowledge the grants from RGC of HKSAR project number T42-103/16N.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vellaisamy, Professor Roy
Authors: Oo, S. L., Ilyas, A.-M., Karthikeyan, V., Arava, C. M., Kong, E. Y., Yeung, C.-C., Chen, X., Yu, P. K.N., and Vellaisamy, R. A.L.
College/School:College of Science and Engineering > School of Engineering
Journal Name:Analytical Chemistry
Publisher:American Chemical Society
ISSN:0003-2700
ISSN (Online):1520-6882
Published Online:06 January 2020
Copyright Holders:Copyright © 2020 American Chemical Society
First Published:First published in Analytical Chemistry 92(2):1738-1745
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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