Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells

Sepp, K., Lee, M., Bluntzer, M., Helgason, G. V. , Hulme, A. N. and Brunton, V. G. (2020) Utilizing stimulated Raman scattering microscopy to study intracellular distribution of label-free ponatinib in live cells. Journal of Medicinal Chemistry, 63(5), pp. 2028-2034. (doi: 10.1021/acs.jmedchem.9b01546) (PMID:31829628) (PMCID:PMC7073915)

Abstract

Stimulated Raman scattering (SRS) microscopy represents a powerful method for imaging label-free drug dis-tribution with high resolution. SRS was applied to image label-free ponatinib with high sensitivity and speci-ficity in live human chronic myeloid leukemia (CML) cell lines. This was achieved at biologically relevant, na-nomolar concentrations; allowing determination of ponatinib uptake and sequestration into lysosomes during the development of acquired drug resistance and an improved understanding of target engagement.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Helgason, Professor Vignir
• Authors: Sepp, K., Lee, M., Bluntzer, M., Helgason, G. V., Hulme, A. N., and Brunton, V. G.
• College/School: College of Medical Veterinary and Life Sciences > School of Cancer Sciences
• Journal Name: Journal of Medicinal Chemistry
• Publisher: American Chemical Society
• ISSN: 0022-2623
• ISSN (Online): 1520-4804
• Published Online: 12 December 2019
• Copyright Holders: Copyright © 2019 American Chemical Society
• First Published: First published in Journal of Medicinal Chemistry 63(5):2028-2034
• Publisher Policy: Reproduced in accordance with the publisher copyright policy