Development of label-free biophysical markers in osteogenic maturation

Bartolozzi, A., Viti, F., De Stefano, S., Sbrana, F., Petecchia, L., Gavazzo, P. and Vassalli, M. (2020) Development of label-free biophysical markers in osteogenic maturation. Journal of the Mechanical Behavior of Biomedical Materials, 103, 103581. (doi: 10.1016/j.jmbbm.2019.103581)

Abstract

The spatial and temporal changes of morphological and mechanical properties of living cells reflect complex functionally-associated processes. Monitoring these modifications could provide a direct information on the cellular functional state. Here we present an integrated biophysical approach to the quantification of the morphological and mechanical phenotype of single cells along a maturation pathway. Specifically, quantitative phase microscopy and single cell biomechanical testing were applied to the characterization of the maturation of human foetal osteoblasts, demonstrating the ability to identify effective label-free biomarkers along this fundamental biological process.

• Item Type: Articles
• Additional Information: This work was supported by the Ministry of Education, Universities and Research of Italy, in the context of the FIRB ‘Futuro in Ricerca 2013’ project [grant number RBFR13V4M2].
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Vassalli, Professor Massimo
• Authors: Bartolozzi, A., Viti, F., De Stefano, S., Sbrana, F., Petecchia, L., Gavazzo, P., and Vassalli, M.
• College/School: College of Science and Engineering > School of Engineering > Biomedical Engineering
• Journal Name: Journal of the Mechanical Behavior of Biomedical Materials
• Publisher: Elsevier
• ISSN: 1751-6161
• ISSN (Online): 1878-0180
• Published Online: 05 December 2019
• Copyright Holders: Copyright © 2019 Elsevier Ltd.
• First Published: First published in Journal of the Mechanical Behavior of Biomedical Materials 103:103581
• Publisher Policy: Reproduced in accordance with the copyright policy of the publisher