Walrad, P. B., Capewell, P. , Fenn, K. and Matthews, K. R. (2012) The post-transcriptional trans-acting regulator, TbZFP3, co-ordinates transmission-stage enriched mRNAs in Trypanosoma brucei. Nucleic Acids Research, 40(7), pp. 2869-2883. (doi: 10.1093/nar/gkr1106) (PMID:22140102) (PMCID:PMC3326296)
|
Text
205118.pdf - Published Version Available under License Creative Commons Attribution Non-commercial. 8MB |
Abstract
Post-transcriptional gene regulation is essential to eukaryotic development. This is particularly emphasized in trypanosome parasites where genes are co-transcribed in polycistronic arrays but not necessarily co-regulated. The small CCCH protein, TbZFP3, has been identified as a trans-acting post-transcriptional regulator of Procyclin surface antigen expression in Trypanosoma brucei. To investigate the wider role of TbZFP3 in parasite transmission, a global analysis of associating transcripts was carried out. Examination of a subset of the selected transcripts revealed their increased abundance through mRNA stabilization upon TbZFP3 ectopic overexpression, dependent upon the integrity of the CCCH zinc finger domain. Reporter assays demonstrated that this regulation was mediated through 3′-UTR sequences for two target transcripts. Global developmental expression profiling of the cohort of TbZFP3-selected transcripts revealed their significant enrichment in transmissible stumpy forms of the parasite. This analysis of the specific mRNAs selected by the TbZFP3mRNP provides evidence for a developmental regulon with the potential to co-ordinate genes important in parasite transmission.
Item Type: | Articles |
---|---|
Additional Information: | Funding: Wellcome Trust (088293, Programme Grant to K.R.M.) (supporting P.B.W, P.C. and K.F.) and Wellcome Trust, The Centre for Immunity, Infection and Evolution (095831, strategic award). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Capewell, Dr Paul |
Authors: | Walrad, P. B., Capewell, P., Fenn, K., and Matthews, K. R. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | Nucleic Acids Research |
Publisher: | Oxford University Press |
ISSN: | 0305-1048 |
ISSN (Online): | 1362-4962 |
Published Online: | 01 December 2011 |
Copyright Holders: | Copyright © 2011 The Authors |
First Published: | First published in Nucleic Acids Research 40(7): 2869-2883 |
Publisher Policy: | Reproduced under a Creative Commons License |
University Staff: Request a correction | Enlighten Editors: Update this record