Analysis of Theileria equi diversity in The Gambia using a novel genotyping method

Coultous, R. , McDonald, M. , Raftery, A. G. , Shiels, B. , Sutton, D. G.M. and Weir, W. (2020) Analysis of Theileria equi diversity in The Gambia using a novel genotyping method. Transboundary and Emerging Diseases, 67(3), pp. 1213-1221. (doi: 10.1111/tbed.13454) (PMID:31845493)

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Abstract

Theileria equi, one of the primary pathogens causing equine piroplasmosis, has previously been sub‐classified into a number of clades on the basis of 18S SSU rRNA gene sequence diversity. This partitioning of the parasite population has potential implications for host immunity, treatment and vaccine development. To detect and identify different clade genotypes among and within individual equine blood samples, a novel PCR‐based technique was designed and optimized. Theileria equi has only recently been described in The Gambia, and the developed genotyping technique was used to analyse blood samples taken from 42 piroplasmosis‐positive horses and donkeys within the country. Three different T. equi genotypes were detected within the population, including the same genotype as the recently described Theileria haneyi, with 61.9% of individuals found to be infected with more than one genotype. Overall, there was a trend that males were more likely to have a multiple genotype infection. Thus, the novel genotyping technique has been shown to be effective in analysis of field populations and offers researchers a rapid method of identifying multiple T. equi genotypes both within individuals and equine populations in epidemiological studies.

Item Type:Articles
Additional Information:The Horserace Betting Levy Board (HBLB) funded the cost of the PCR screening, and RMC was supported by an HBLB research scholarship (VET/RS/254).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Shiels, Professor Brian and Raftery, Alexandra and McDonald, Mr Mike and Sutton, Professor David and Coultous, Dr Robert and Weir, Professor Willie
Authors: Coultous, R., McDonald, M., Raftery, A. G., Shiels, B., Sutton, D. G.M., and Weir, W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:Transboundary and Emerging Diseases
Publisher:Wiley
ISSN:1865-1674
ISSN (Online):1865-1682
Published Online:16 December 2019
Copyright Holders:Copyright © 2019 Blackwell Verlag GmbH
First Published:First published in Transboundary and Emerging Diseases 67(3): 1213-1221
Publisher Policy:Reproduced in accordance with the publisher copyright policy

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190867Equine piroplasmosis: evaluating the threat to the UK and IrelandWilliam WeirHorserace Betting Levy Board (HBLB)VET/RS/254Institute of Biodiversity, Animal Health and Comparative Medicine