Genetic and transcriptional analysis of phosphoinositide-specific phospholipase C in Plasmodium

Raabe, A., Berry, L., Sollelis, L., Cerdan, R., Tawk, L., Vial, H. J., Billker, O. and Wengelnik, K. (2011) Genetic and transcriptional analysis of phosphoinositide-specific phospholipase C in Plasmodium. Experimental Parasitology, 129(1), pp. 75-80. (doi: 10.1016/j.exppara.2011.05.023) (PMID:21651909)

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Abstract

Phosphoinositide-specific phospholipase C (PI-PLC) is a major regulator of calcium-dependent signal transduction, which has been shown to be important in various processes of the malaria parasite Plasmodium. PI-PLC is generally implicated in calcium liberation from intracellular stores through the action of its product, inositol-(1,4,5)-trisphosphate, and is itself dependent on calcium for its activation. Here we describe the plc genes from Plasmodium species. The encoded proteins contain all domains typically found in PI-PLCs of the δ class but are almost twice as long as their orthologues in mammals. Transcriptional analysis by qRT-PCR of plc during the erythrocytic cycle of P. falciparum revealed steady expression levels that increased at the late schizont stages. Genetic analysis in the P. berghei model revealed that the plc locus was targetable but that plc gene knock-outs could not be obtained, thereby strongly indicating that the gene is essential during blood stage development. Alternatively, we attempted to modify plc expression through a promoter exchange approach but found the gene to be refractory to over-expression indicating that plc expression levels might additionally be tightly controlled.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sollelis, Miss Lauriane
Authors: Raabe, A., Berry, L., Sollelis, L., Cerdan, R., Tawk, L., Vial, H. J., Billker, O., and Wengelnik, K.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Experimental Parasitology
Publisher:Elsevier
ISSN:0014-4894
ISSN (Online):1090-2449
Copyright Holders:Copyright © 2011 Elsevier Inc.
First Published:First published in Experimental Parasitology 129(1):75-80
Publisher Policy:Reproduced under a Creative Commons License

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