Cardiac SNARE expression in health and disease

Gould, G. , Bowman, P. and Smith, G. (2019) Cardiac SNARE expression in health and disease. Frontiers in Endocrinology, 10, 881. (doi: 10.3389/fendo.2019.00881) (PMID:31920989) (PMCID:PMC6930865)

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Abstract

SNARE proteins are integral to intracellular vesicular trafficking, which in turn is the process underlying the regulated expression of substrate transporters such as the glucose transporter GLUT4 at the cell surface of insulin target tissues. Impaired insulin stimulated GLUT4 trafficking is associated with reduced cardiac function in many disease states, most notably diabetes. Despite this, our understanding of the expression and regulation of SNARE proteins in cardiac tissue and how these may change in diabetes is limited. Here we characterize the array of SNARE proteins expressed in cardiac tissue, and quantify the levels of expression of VAMP2, SNAP23, and Syntaxin4—key proteins involved in insulin-stimulated GLUT4 translocation. We examined SNARE protein levels in cardiac tissue from two rodent models of insulin resistance, db/db mice and high-fat fed mice, and show alterations in patterns of expression are evident. Such changes may have implications for cardiac function.

Item Type:Articles
Additional Information:This work was supported by a Ph.D. scholarship to PRTB (FS/14/61/31284) and project grants (PG/18/47/33822) from The British Heart Foundation to GG and GS, and Diabetes UK (15/0005246 and 13/0004696 to GG).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Gould, Professor Gwyn and Bowman, Mr Peter and Smith, Professor Godfrey
Authors: Gould, G., Bowman, P., and Smith, G.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Frontiers in Endocrinology
Publisher:Frontiers Media
ISSN:1664-2392
ISSN (Online):1664-2392
Copyright Holders:Copyright © 2019 Bowman, Smith and Gould
First Published:First published in Frontiers in Endocrinology 10:881
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190812BHF 4 Year PhD Studentship AwardRhian TouyzBritish Heart Foundation (BHF)FS/14/61/31284Institute of Cardiovascular & Medical Sciences
303465Development of a model of insulin-stimulated glucose transport in human cardiomyocytesGwyn GouldBritish Heart Foundation (BHF)PG/18/47/33822Institute of Molecular, Cell & Systems Biology
173377How does Cholesterol regulate insulin action in adipocytes?Gwyn GouldDiabetes UK (DIABETUK)15/0005246Institute of Molecular, Cell & Systems Biology
169439Investigation of EFR3's role in regulating phosphatidyl-inositol-4-phosphate patches in the plasma membrane of adipocytes to mediate insulin-dependent dispersal of GLUT4 clustersGwyn GouldDiabetes UK (DIABETUK)Bryant, Prof Nia 13/00046Institute of Molecular, Cell & Systems Biology