Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates

Allkja, J. et al. (2020) Minimum information guideline for spectrophotometric and fluorometric methods to assess biofilm formation in microplates. Biofilm, 2, 100010. (doi: 10.1016/j.bioflm.2019.100010)

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The lack of reproducibility of published studies is one of the major issues facing the scientific community, and the field of biofilm microbiology has been no exception. One effective strategy against this multifaceted problem is the use of minimum information guidelines. This strategy provides a guide for authors and reviewers on the necessary information that a manuscript should include for the experiments in a study to be clearly interpreted and independently reproduced. As a result of several discussions between international groups working in the area of biofilms, we present a guideline for the spectrophotometric and fluorometric assessment of biofilm formation in microplates. This guideline has been divided into 5 main sections, each presenting a comprehensive set of recommendations. The intention of the minimum information guideline is to improve the quality of scientific communication that will augment interlaboratory reproducibility in biofilm microplate assays.

Item Type:Articles
Additional Information:This project has received funding from the European Union’s Horizon 2020 research and innovation program under the Marie Sklodowska – Curie grant agreement No 722467, as part of the Print-Aid consortium. This work received additional financial support by: project UID/EQU/00511/2019 - Laboratory for Process Engineering, Environment, Biotechnology and Energy – LEPABE funded by national funds through FCT/MCTES (PIDDAC); Project “LEPABE-2-ECO-INNOVATION” – NORTE‐01‐0145‐FEDER‐000005, funded by Norte Portugal Regional Operational Programme (NORTE 2020), under PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF).
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon
Authors: Allkja, J., Bjarnsholt, T., Coenye, T., Cos, P., Fallarero, A., Harrison, J. J., Lopes, S. P., Oliver, A., Pereira, M. O., Ramage, G., Shirtliff, M. E., Stoodley, P., Webb, J. S., Zaat, S. A.J., Goeres, D. M., and Azevedo, N. F.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Biofilm
ISSN (Online):2590-2075
Published Online:19 November 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Biofilm 2: 100010
Publisher Policy:Reproduced under a Creative Commons License

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