Clarke, C., Woods, R.J., Gluska, J., Cooper, A., Nutley, M.A. and Boons, G.J. (2001) Involvement of water in carbohydrate-protein binding. Journal of the American Chemical Society, 123(49), pp. 12238-12247. (doi: 10.1021/ja004315q)
Full text not currently available from Enlighten.
Publisher's URL: http://dx.doi.org/10.1021/ja004315q
Abstract
The interactions of trimannosides 1 and 2 with Con A were studied to reveal the effects of displacement of well-ordered water molecules on the thermodynamic parameters of protein- ligand complexation. Trisaccharide 2 is a derivative of 1. in which the hydroxyl at C-2 of the central mannose unit is replaced by a hydroxyethyl moiety. Upon binding, this moiety displaces a conserved water molecule present in the Con A binding site. Structural studies by NMR spectroscopy and MD simulations showed that the two compounds have very similar solution conformational properties. MD simulations of the complexes of Con A with 1 and 2 demonstrated that the hydroxyethyl side chain of 2 can establish the same hydrogen bonds in a low energy conformation with the protein binding site as those mediated by the water molecule in the complex of 1 with Con A. Isothermal titration microcalorimetry (ITC) measurements showed that 2 has a more favorable entropy of binding compared to 1. This term, which was expected, arises from the return of the highly ordered water molecule to bulk solution. The favorable entropy term was, however, offset by a relatively large unfavorable enthalpy term. This observation was rationalized by comparing the extent of hydrogen bond and solvation changes during binding. It is proposed that an indirect interaction through a water molecule will provide a larger number of hydrogen bonds in the complex that have higher occupancies than in bulk solution, thereby stabilizing the complex.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Nutley, Mrs Margaret and Cooper, Professor Alan |
Authors: | Clarke, C., Woods, R.J., Gluska, J., Cooper, A., Nutley, M.A., and Boons, G.J. |
Subjects: | Q Science > QD Chemistry Q Science > QH Natural history > QH345 Biochemistry |
College/School: | College of Science and Engineering > School of Chemistry |
Journal Name: | Journal of the American Chemical Society |
Publisher: | American Chemical Society |
ISSN: | 0002-7863 |
ISSN (Online): | 1520-5126 |
Published Online: | 10 November 2001 |
University Staff: Request a correction | Enlighten Editors: Update this record