The DvA-1 polyprotein of the parasitic nematode Dictyocaulus viviparus. A small helix-rich lipid-binding protein

Kennedy, M. W. , Britton, C. , Price, N. C., Kelly, S. M. and Cooper, A. (1995) The DvA-1 polyprotein of the parasitic nematode Dictyocaulus viviparus. A small helix-rich lipid-binding protein. Journal of Biological Chemistry, 270(33), pp. 19277-19281. (doi: 10.1074/jbc.270.33.19277) (PMID:7642601)

Abstract

DNA encoding a single unit of the DvA-1 polyprotein of the parasitic nematode Dictyocaulus viviparus was isolated and the polypeptide ("rDvA-1L") expressed in Escherichia coli, to give a protein showing high binding affinity for fatty acids and retinoids. Fluorescent fatty acid probes show substantial changes in emission spectrum in the presence of rDvA-1L, which can be reversed by fatty acids (oleic, palmitic, stearic, arachidonic) and retinoids, but not by tryptophan, squalene, or cholesterol. Moreover, changes in intrinsic fluorescence of retinol or retinoic acid confirm a retinoid binding activity. Fluorescence titration experiments indicate stoichiometric binding to a single protein site per monomer unit with affinities (Kd) in the range 3 x 10(-8) M for 11-((5-dimethylaminonaphthalene-1-sulfonyl)amino)undecanoic acid, and by competition, 5 x 10(-8) M for oleic acid. The extreme blue shift of bound fluorescent fatty acid suggests an unusually low polarity for the protein binding site. The emission spectrum of the single tryptophan of rDvA-1L indicates that it is deeply buried in a nonpolar environment, and its spectrum is unaffected by ligand binding. Far UV circular dichroism of rDvA-1L reveals a high alpha-helix content (53%). Differential scanning calorimetry studies indicate that rDvA-1L is highly stable (T(m) approximately 98 degrees C), refolding efficiently following thermal denaturation. DvA-1 therefore represents an example of a new class of lipid binding protein, and is the first product of a polyprotein with this activity to be described.

• Item Type: Articles
• Additional Information: This work was supported in part by Wellcome Trust Grant 031559/Z/90/A (to M. W. K.).
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kennedy, Professor Malcolm and Kelly, Dr Sharon and Cooper, Professor Alan and Britton, Professor Collette
• Authors: Kennedy, M. W., Britton, C., Price, N. C., Kelly, S. M., and Cooper, A.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences; College of Science and Engineering > School of Chemistry; College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
• Journal Name: Journal of Biological Chemistry
• Publisher: American Society for Biochemistry and Molecular Biology, Inc.
• ISSN: 0021-9258
• ISSN (Online): 1083-351X