Controlled single-cell cyclic compression and transcription analysis: a pilot study

Gavazzo, P., Petecchia, L., Facci, P., Vassalli, M. and Viti, F. (2017) Controlled single-cell cyclic compression and transcription analysis: a pilot study. Biophysical Chemistry, 229, pp. 39-45. (doi: 10.1016/j.bpc.2017.07.010) (PMID:28818313)

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Abstract

An innovative platform for the study of the molecular mechanisms at the basis of mechanotransduction has been implemented, developing an experimental approach capable of providing controlled dynamic compression stimuli and retrieving the biomolecular response with single-cell sensitivity. The system provides the ability to perform compression-release cycles on single cells with controlled forces in the nN range and a user-defined repetition rate. Experimental procedures to perform qPCR from a small set of single cells were finely tuned. The experimental platform was tested in the context of bone (cell line hFOB 1.19), a physiological environment highly subjected to mechanical stimuli. Target genes were identified in the literature, based on their involvement in the osteogenesis process or in the bone response to mechanical stimuli. qPCR analysis shows an increase in expression of the chosen targets, and confirms the effectiveness of the presented approach for studying living single cells response to dynamic compression.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Vassalli, Dr Massimo
Authors: Gavazzo, P., Petecchia, L., Facci, P., Vassalli, M., and Viti, F.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Biophysical Chemistry
Publisher:Elsevier
ISSN:0301-4622
ISSN (Online):1873-4200
Published Online:12 August 2017

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