Simultaneous quantification of estrogens and glucocorticoids in human adipose tissue by liquid-chromatography-tandem mass spectrometry

Laforest, S. et al. (2019) Simultaneous quantification of estrogens and glucocorticoids in human adipose tissue by liquid-chromatography-tandem mass spectrometry. Journal of Steroid Biochemistry and Molecular Biology, 195, 105476. (doi: 10.1016/j.jsbmb.2019.105476) (PMID:31561001)

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Abstract

The presence of estrogens, androgens and glucocorticoids as well as their receptors and steroid converting enzymes in adipose tissue has been established. Their contribution to diseases such as obesity, diabetes and hormone-dependent cancers is an active area of research. Our objective was to develop a LC–MS/MS method to quantify bioactive estrogens and glucocorticoids simultaneously in human adipose tissue. Estrogens and glucocorticoids were extracted from adipose tissue samples using solid-phase extraction. Estrogens were derivatized using 1-(2,4-dinitro-5-fluorophenyl)-4-methylpiperazine (PPZ) and methyl iodide to generate a permanently charged molecule (MPPZ). Steroids were separated and quantified by LC–MS/MS. The limit of quantitation for the steroids was between 15 and 100 pg per sample. Accuracy and precision were acceptable (<20%). Using this method, estradiol, estrone, cortisone and cortisol were quantified in adipose tissue from women with and without breast cancer. This novel assay of estrogens and glucocorticoids by LC–MS/MS coupled with derivatization allowed simultaneous quantification of a panel of steroids in human adipose tissue across the endogenous range of concentrations encountered in health and disease.

Item Type:Articles
Additional Information:The study was supported by operating funds from the CMDO to AT. SL is the recipient of PhD scholarships from Fonds de recherche du Québec-santé (FRQS) and the Canadian Institutes of Health Research (CIHR). SL’s internship in RA’s lab was made possible thanks to the support of the Institute of Nutrition and Functional Foods and the scholarship from the International internship program of the Fonds de recherche Nature et Technologies du Québec (FRQNT) as well as a Michael Smith Foreign Study Supplement travel scholarship. RA is funded by the Wellcome Trust. BRW is a Wellcome Trust Senior Investigator. CD holds an Investigator Awards (Senior) from the FRQS. ND is funded by a BBSRC iCASE award (BB/N503691/1).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Denver, Nina
Authors: Laforest, S., Pelletier, M., Denver, N., Poirier, B., Nguyen, S., Walker, B. R., Durocher, F., Homer, N. Z.M., Diorio, C., Tchernof, A., and Andrew, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Journal of Steroid Biochemistry and Molecular Biology
Publisher:Elsevier
ISSN:0960-0760
ISSN (Online):1879-1220
Published Online:24 September 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Journal of Steroid Biochemistry and Molecular Biology 195:105476
Publisher Policy:Reproduced under a Creative Commons License

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