Robertson, S., Childs, P. G., Akinbobola, A., Henriquez, F. L., Ramage, G. , Reid, S., Mackay, W. G. and Williams, C. (2020) Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration. Journal of Bioscience and Bioengineering, 129(3), pp. 379-386. (doi: 10.1016/j.jbiosc.2019.09.003) (PMID:31623950)
Text
202142.pdf - Accepted Version Available under License Creative Commons Attribution Non-commercial No Derivatives. 813kB | ||
|
Text
202142Suppl.pdf - Supplemental Material 539kB |
Abstract
Bacterial biofilms pose a significant burden in both healthcare and industrial environments. With the limited effectiveness of current biofilm control strategies, novel or adjunctive methods in biofilm control are being actively pursued. Reported here, is the first evidence of the application of nanovibrational stimulation (nanokicking) to reduce the biofilm formation of Pseudomonas aeruginosa. Nanoscale vertical displacements (approximately 60 nm) were imposed on P. aeruginosa cultures, with a significant reduction in biomass formation observed at frequencies between 200 and 4000 Hz at 24 h. The optimal reduction of biofilm formation was observed at 1 kHz, with changes in the physical morphology of the biofilms. Scanning electron microscope imaging of control and biofilms formed under nanovibrational stimulation gave indication of a reduction in extracellular matrix (ECM). Quantification of the carbohydrate and protein components of the ECM was performed and showed a significant reduction at 24 h at 1 kHz frequency. To model the forces being exerted by nanovibrational stimulation, laser interferometry was performed to measure the amplitudes produced across the Petri dish surfaces. Estimated peak forces on each cell, associated with the nanovibrational stimulation technique, were calculated to be in the order of 10 pN during initial biofilm formation. This represents a potential method of controlling microbial biofilm formation in a number of important settings in industry and medical related processes.
Item Type: | Articles |
---|---|
Additional Information: | Funding: EPSRC (EP/N012631/1). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Ramage, Professor Gordon and Henriquez, Dr Fiona and Childs, Dr Peter |
Authors: | Robertson, S., Childs, P. G., Akinbobola, A., Henriquez, F. L., Ramage, G., Reid, S., Mackay, W. G., and Williams, C. |
College/School: | College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School College of Science and Engineering > School of Engineering > Biomedical Engineering |
Journal Name: | Journal of Bioscience and Bioengineering |
Publisher: | Elsevier |
ISSN: | 1389-1723 |
ISSN (Online): | 1389-1723 |
Published Online: | 15 October 2019 |
Copyright Holders: | Copyright © 2019 The The Society for Biotechnology, Japan |
First Published: | First published in Journal of Bioscience and Bioengineering 129(3): 379-386 |
Publisher Policy: | Reproduced in accordance with the copyright policy of the publisher |
University Staff: Request a correction | Enlighten Editors: Update this record