Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration

Robertson, S., Childs, P. G., Akinbobola, A., Henriquez, F. L., Ramage, G. , Reid, S., Mackay, W. G. and Williams, C. (2020) Reduction of Pseudomonas aeruginosa biofilm formation through the application of nanoscale vibration. Journal of Bioscience and Bioengineering, 129(3), pp. 379-386. (doi: 10.1016/j.jbiosc.2019.09.003) (PMID:31623950)

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Abstract

Bacterial biofilms pose a significant burden in both healthcare and industrial environments. With the limited effectiveness of current biofilm control strategies, novel or adjunctive methods in biofilm control are being actively pursued. Reported here, is the first evidence of the application of nanovibrational stimulation (nanokicking) to reduce the biofilm formation of Pseudomonas aeruginosa. Nanoscale vertical displacements (approximately 60 nm) were imposed on P. aeruginosa cultures, with a significant reduction in biomass formation observed at frequencies between 200 and 4000 Hz at 24 h. The optimal reduction of biofilm formation was observed at 1 kHz, with changes in the physical morphology of the biofilms. Scanning electron microscope imaging of control and biofilms formed under nanovibrational stimulation gave indication of a reduction in extracellular matrix (ECM). Quantification of the carbohydrate and protein components of the ECM was performed and showed a significant reduction at 24 h at 1 kHz frequency. To model the forces being exerted by nanovibrational stimulation, laser interferometry was performed to measure the amplitudes produced across the Petri dish surfaces. Estimated peak forces on each cell, associated with the nanovibrational stimulation technique, were calculated to be in the order of 10 pN during initial biofilm formation. This represents a potential method of controlling microbial biofilm formation in a number of important settings in industry and medical related processes.

Item Type:Articles
Additional Information:Funding: EPSRC (EP/N012631/1).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Ramage, Professor Gordon and Henriquez, Dr Fiona and Childs, Dr Peter
Authors: Robertson, S., Childs, P. G., Akinbobola, A., Henriquez, F. L., Ramage, G., Reid, S., Mackay, W. G., and Williams, C.
College/School:College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:Journal of Bioscience and Bioengineering
Publisher:Elsevier
ISSN:1389-1723
ISSN (Online):1389-1723
Published Online:15 October 2019
Copyright Holders:Copyright © 2019 The The Society for Biotechnology, Japan
First Published:First published in Journal of Bioscience and Bioengineering 129(3): 379-386
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172535Development of NanoKick BioreactorMatthew DalbyBiotechnology and Biological Sciences Research Council (BBSRC)BB/N012690/1Institute of Molecular, Cell & Systems Biology