Influence of nitric oxide on luminol-enhanced chemiluminescence measured from porcine-stimulated leukocytes

Demiryürek, A.T., Kennedy, S. , Wainwright, C. L., Wadsworth, R.M. and Kane, K.A. (1997) Influence of nitric oxide on luminol-enhanced chemiluminescence measured from porcine-stimulated leukocytes. Journal of Cardiovascular Pharmacology, 30(3), pp. 332-337. (doi: 10.1097/00005344-199709000-00010) (PMID:9300317)

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The influence of endogenous nitric oxide (NO) and NO-releasing compounds on free radical release from porcine leukocytes was investigated by luminol-enhanced chemiluminescence (CL). The direct free radical-scavenging activity of the compounds was determined by a cell-free system using xanthine plus xanthine oxidase (X+XO). The NO donor, N-(2-hydroxyethyl)nicotinamide nitrate (nicorandil), markedly inhibited CL generated by phorbol myristate acetate (PMA)-stimulated leukocytes. In addition, nicorandil and S-nitrozo-N-acetylpenicillamine (SNAP) both decreased CL generated by X+XO. Conversely, C87 3754, a NO-releasing sydnonimine, decreased free radical release from leukocytes only when preincubated with the cells and had no effects on the X+XO system. None of the NO donors inhibited peroxynitrite-generated CL. L-, but not D-, arginine inhibited PMA-activated free radical generation without affecting X+XO-induced CL. L-Canavanine, Nω-nitro-L-arginine (L-NNA), and L-nitro-arginine methyl ester (L-NAME), inhibitors of the NO pathway, augmented PMA-induced CL. However, L-canavanine, but not L-NNA and L-NAME, produced a significant inhibition of X+XO-induced CL. It is concluded that endogenous NO may play an important role in the measurement of free radicals released from porcine leukocytes, assessed by luminol-enhanced CL, and that compounds with NO-releasing properties decrease CL, possibly by interfering with free radical generation.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Kennedy, Professor Simon
Authors: Demiryürek, A.T., Kennedy, S., Wainwright, C. L., Wadsworth, R.M., and Kane, K.A.
College/School:College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Journal of Cardiovascular Pharmacology
Publisher:Lippincott, Williams & Wilkins
ISSN (Online):1533-4023

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