Impedimetric detection and electromediated apoptosis of vascular smooth muscle using microfabricated biosensors for diagnosis and therapeutic treatment in cardiovascular diseases

Bussooa, A., Hoare, D., Kirimi, M. T., Mitra, S., Mirzai, N., Neale, S. L. and Mercer, J. (2020) Impedimetric detection and electromediated apoptosis of vascular smooth muscle using microfabricated biosensors for diagnosis and therapeutic treatment in cardiovascular diseases. Advanced Science, 7(18), 1902999. (doi: 10.1002/advs.201902999) (PMID:32999823) (PMCID:PMC7509665)

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Abstract

Cardiovascular diseases remain a significant global burden with 1‐in‐3 of all deaths attributable to the consequences of the disease. The main cause is blocked arteries which often remain undetected. Implantable medical devices (IMDs) such as stents and grafts are often used to reopen vessels but over time these too will re‐block. A vascular biosensor is developed that can report on cellularity and is amenable to being mounted on a stent or graft for remote reporting. Moreover, the device is designed to also receive currents that can induce a controlled form of cell death, apoptosis. A combined diagnostic and therapeutic biosensor would be transformational for the treatment of vascular diseases such as atherosclerosis and central line access. In this work, a cell sensing and cell apoptosing system based on the same interdigitated electrodes (IDEs) is developed. It is shown that the device is scalable and that by miniaturizing the IDEs, the detection sensitivity is increased. Apoptosis of vascular smooth muscle cells is monitored using continuous impedance measurements at a frequency of 10 kHz and rates of cell death are tracked using fluorescent dyes and live cell imaging.

Item Type:Articles
Additional Information:This work was supported by the BHF Centre of Research Excellence RE/13/5/30177, University of Glasgow, College of Medical, Veterinary and Life Sciences, Chief Science Office, Scotland Award (CSO): CGA/17/29 and a Medical Research Council Confidence in Concept (MRC-CiC) award.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Neale, Dr Steven and Bussooa, Anubhav and Hoare, Daniel and Mercer, Dr John and Kirimi, Mahmut Talha and Mirzai, Mr Nosrat and Mitra, Dr Srinjoy
Authors: Bussooa, A., Hoare, D., Kirimi, M. T., Mitra, S., Mirzai, N., Neale, S. L., and Mercer, J.
College/School:College of Medical Veterinary and Life Sciences
College of Science and Engineering > School of Engineering
College of Science and Engineering > School of Engineering > Systems Power and Energy
Journal Name:Advanced Science
Publisher:Wiley
ISSN:2198-3844
ISSN (Online):2198-3844
Published Online:27 July 2020
Copyright Holders:Copyright © 2020 The Authors
First Published:First published in Advanced Science 7(18):1902999
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190814BHF centre of excellenceRhian TouyzBritish Heart Foundation (BHF)RE/13/5/30177Institute of Cardiovascular & Medical Sciences
301336Design and fabrication of a remote controlled wireless impedance sensing unit for a new cardiovascular medical device.John MercerChief Scientist Office (CSO)CGA/17/29CAMS - Cardiovascular Science