Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR

Wells, B. et al. (2015) Molecular detection of Toxoplasma gondii in water samples from Scotland and a comparison between the 529bp real-time PCR and ITS1 nested PCR. Water Research, 87, pp. 175-181. (doi: 10.1016/j.watres.2015.09.015) (PMID:26408950)

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Abstract

Waterborne transmission of Toxoplasma gondii is a potential public health risk and there are currently no agreed optimised methods for the recovery, processing and detection of T. gondii oocysts in water samples. In this study modified methods of T. gondii oocyst recovery and DNA extraction were applied to 1427 samples collected from 147 public water supplies throughout Scotland. T. gondii DNA was detected, using real time PCR (qPCR) targeting the 529bp repeat element, in 8.79% of interpretable samples (124 out of 1411 samples). The samples which were positive for T. gondii DNA originated from a third of the sampled water sources. The samples which were positive by qPCR and some of the negative samples were reanalysed using ITS1 nested PCR (nPCR) and results compared. The 529bp qPCR was the more sensitive technique and a full analysis of assay performance, by Bayesian analysis using a Markov Chain Monte Carlo method, was completed which demonstrated the efficacy of this method for the detection of T. gondii in water samples.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Innes, Professor Elisabeth and Hotchkiss, Dr Emily
Authors: Wells, B., Shaw, H., Innocent, G., Guido, S., Hotchkiss, E., Parigi, M., Opsteegh, M., Green, J., Gillespie, S., Innes, E. A., and Katzer, F.
College/School:College of Medical Veterinary and Life Sciences > School of Infection & Immunity
College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine
Journal Name:Water Research
Publisher:Elsevier
ISSN:0043-1354
ISSN (Online):1879-2448
Published Online:09 September 2015
Copyright Holders:Copyright © 2015 The Authors
First Published:First published in Water Research 87: 175-181
Publisher Policy:Reproduced under a Creative Commons License

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