Environmental contamination and hygienic measures after feline calicivirus field strain infections of cats in a research facility

Spiri, A. M., Meli, M. L., Riond, B., Herbert, I., Hosie, M. J. and Hofmann-Lehmann, R. (2019) Environmental contamination and hygienic measures after feline calicivirus field strain infections of cats in a research facility. Viruses, 11(10), 958. (doi: 10.3390/v11100958)

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Abstract

Feline calicivirus (FCV) can cause painful oral ulcerations, salivation, gingivitis/stomatitis, fever and depression in infected cats; highly virulent virus variants can lead to fatal epizootic outbreaks. Viral transmission occurs directly or indirectly via fomites. The aim of this study was to investigate the presence and viability of FCV in the environment after sequential oronasal infections of specified pathogen-free cats with two FCV field strains in a research facility. Replicating virus was detected in saliva swabs from all ten cats after the first and in four out of ten cats after the second FCV exposure using virus isolation to identify FCV shedders. In the environment, where cleaning, but no disinfection took place, FCV viral RNA was detectable using RT-qPCR on all tested items and surfaces, including cat hair. However, only very limited evidence was found of replicating virus using virus isolation. Viral RNA remained demonstrable for at least 28 days after shedding had ceased in all cats. Disinfection with 5% sodium bicarbonate (and IncidinTM Plus) and barrier measures were effective in that no viral RNA was detectable outside the cat rooms. Our findings are important for any multicat environment to optimize hygienic measures against FCV infection.

Item Type:Articles
Additional Information:Funding: A.M.S. was partly funded by the research grant (Forschungskredit) of the University of Zurich, grant number FK-53210-01-01 and by the research grant from the foundation “Stiftung für Kleintiere” of the Vetsuisse Faculty, University of Zurich. I.H. and M.J.H. were funded by an Industrial Partnership Ph.D. award from the University of Glasgow and Merial.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Hosie, Professor Margaret and Herbert, Imogen
Authors: Spiri, A. M., Meli, M. L., Riond, B., Herbert, I., Hosie, M. J., and Hofmann-Lehmann, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Viruses
Publisher:MDPI
ISSN:1999-4915
ISSN (Online):1999-4915
Published Online:17 October 2019
Copyright Holders:Copyright © 2019 by the authors
First Published:First published in Viruses 11(10):958
Publisher Policy:Reproduced under a Creative Commons license

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