Norton, N. J., Fun, A., Bandara, M. , Wills, M. R., Mok, H. P. and Lever, A. M.L. (2017) Innovations in the quantitative virus outgrowth assay and its use in clinical trials. Retrovirology, 14, 58. (doi: 10.1186/s12977-017-0381-2) (PMID:29268753) (PMCID:PMC5740843)
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Abstract
A robust measure of the size of the latent HIV reservoir is essential to quantifying the effect of interventions designed to deplete the pool of reactivatable, replication competent proviruses. In addition to the ability to measure a biologically relevant parameter, any assay designed to be used in a clinical trial needs to be reproducible and scalable. The need to quantify the number of resting CD4+ T cells capable of releasing infectious virus has led to the development of the quantitative viral outgrowth assay (VOA). The assay as originally described has a number of features that limit its scalability for use in clinical trials; however recent developments reducing the time and manpower requirements of the assay, while importantly improving reproducibility mean that it is becoming much more practical for it to enter into more widespread use. This review describes the background to VOA development and the practical issues that they present in utilising them in clinical trials. It describes the innovations that have made their usage more practical and the limitations that still exist.
| Item Type: | Articles |
|---|---|
| Additional Information: | HPM and NJN are both supported by the Medical Research Council, UK (MR/ N02043X/1 and MR/M003515/1 respectively). Work in the laboratory is supported by the Medical Research Council (UK), the Biomedical Research Centre and the Cambridge Clinical Academic Reserve. |
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Jayaweera Bandara, Dr Mikaila |
| Authors: | Norton, N. J., Fun, A., Bandara, M., Wills, M. R., Mok, H. P., and Lever, A. M.L. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Retrovirology |
| Publisher: | BioMed Central |
| ISSN: | 1742-4690 |
| ISSN (Online): | 1742-4690 |
| Copyright Holders: | Copyright © 2017 The Authors |
| First Published: | First published in Retrovirology 14: 58 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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