Protease-associated import systems are widespread in Gram-negative bacteria

Grinter, R., Leung, P. M., Wijeyewickrema, L. C., Littler, D., Beckham, S., Pike, R. N., Walker, D. , Greening, C. and Lithgow, T. (2019) Protease-associated import systems are widespread in Gram-negative bacteria. PLoS Genetics, 15(10), e1008435. (doi:10.1371/journal.pgen.1008435)

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Abstract

Bacteria have evolved sophisticated uptake machineries in order to obtain the nutrients required for growth. Gram-negative plant pathogens of the genus Pectobacterium obtain iron from the protein ferredoxin, which is produced by their plant hosts. This iron-piracy is mediated by the ferredoxin uptake system (Fus), a gene cluster encoding proteins that transport ferredoxin into the bacterial cell and process it proteolytically. In this work we show that gene clusters related to the Fus are widespread in bacterial species. Through structural and biochemical characterisation of the distantly related Fus homologues YddB and PqqL from Escherichia coli, we show that these proteins are analogous to components of the Fus from Pectobacterium. The membrane protein YddB shares common structural features with the outer membrane ferredoxin transporter FusA, including a large extracellular substrate binding site. PqqL is an active protease with an analogous periplasmic localisation and iron-dependent expression to the ferredoxin processing protease FusC. Structural analysis demonstrates that PqqL and FusC share specific features that distinguish them from other members of the M16 protease family. Taken together, these data provide evidence that protease associated import systems analogous to the Fus are widespread in Gram-negative bacteria.

Item Type:Articles
Additional Information:Funding: The work was funded by the Australian Research Council (ARC; FL130100038) https:// www.arc.gov.au/. R.G. was funded by a Sir Henry Wellcome Fellowship award (106077/Z/14/Z) https://wellcome.ac.uk/. T.L. is an ARC Australian Laureate Fellow (FL130100038).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Walker, Professor Daniel
Creator Roles:
Walker, D.Methodology, Writing – review and editing
Authors: Grinter, R., Leung, P. M., Wijeyewickrema, L. C., Littler, D., Beckham, S., Pike, R. N., Walker, D., Greening, C., and Lithgow, T.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:PLoS Genetics
Publisher:Public Library of Science
ISSN:1553-7390
ISSN (Online):1553-7404
Published Online:15 October 2019
Copyright Holders:Copyright © 2019 Grinter et al.
First Published:First published in PloS Genetics 15(10):e1008435
Publisher Policy:Reproduced under a Creative Commons license

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