Crystallization and preliminary X-ray diffraction studies of 6-phosphogluconate dehydrogenase from Lactococcus lactis

Tetaud, E., Hall, D.R., Gourley, D.G., Leonard, G.A., Arkison, S. , Barrett, M.P. and Hunter, W.N. (1998) Crystallization and preliminary X-ray diffraction studies of 6-phosphogluconate dehydrogenase from Lactococcus lactis. Acta Crystallographica Section D: Biological Crystallography, 54(6.2), pp. 1422-1424. (doi:10.1107/s0907444998004909) (PMID:10089526)

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Abstract

6-Phosphogluconate dehydrogenase is one of the seven enzymes involved in the pentose phosphate pathway. Crystals of a mammalian and a protozoan enzyme have been obtained previously and structures determined. It is reported here that a bacterial 6-phosphogluconate dehydrogenase, from Lactococcus lactis, has been purified and used in crystallization trials. Large prisms suitable for a detailed structural analysis have been obtained and characterized as orthorhombic, space group F222, with a = 70.4, b = 105.7, c = 474.6 Å. Diffraction has been observed to 2.2 Å resolution using synchrotron radiation. Structural analysis, in combination with ongoing biochemical characterization, will assist the elucidation of the structure–activity relationships of this enzyme.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Arkison, Mr Scott and Barrett, Professor Michael
Authors: Tetaud, E., Hall, D.R., Gourley, D.G., Leonard, G.A., Arkison, S., Barrett, M.P., and Hunter, W.N.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Acta Crystallographica Section D: Biological Crystallography
Publisher:International Union of Crystallography
ISSN:0907-4449
ISSN (Online):1399-0047

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