Brown, J. L. , Johnston, W., Delaney, C., Rajendran, R., Butcher, J., Khan, S., Bradshaw, D., Ramage, G. and Culshaw, S. (2019) Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells. Scientific Reports, 9, 15779. (doi: 10.1038/s41598-019-52115-7) (PMID:31673005) (PMCID:PMC6823452)
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Abstract
The gingival epithelium is a physical and immunological barrier to the microbiota of the oral cavity, which interact through soluble mediators with the immune cells that patrol the tissue at the gingival epithelium. We sought to develop a three-dimensional gingivae-biofilm interface model using a commercially available gingival epithelium to study the tissue inflammatory response to oral biofilms associated with “health”, “gingivitis” and “periodontitis”. These biofilms were developed by sequential addition of microorganisms to mimic the formation of supra- and sub-gingival plaque in vivo. Secondly, to mimic the interactions between gingival epithelium and immune cells in vivo, we integrated peripheral blood mononuclear cells and CD14+ monocytes into our three-dimensional model and were able to assess the inflammatory response in the immune cells cultured with and without gingival epithelium. We describe a differential inflammatory response in immune cells cultured with epithelial tissue, and more so following incubation with epithelium stimulated by “gingivitis-associated” biofilm. These results suggest that gingival epithelium-derived soluble mediators may control the inflammatory status of immune cells in vitro, and therefore targeting of the epithelial response may offer novel therapies. This multi-cellular interface model, both of microbial and host origin, offers a robust in vitro platform to investigate host-pathogens at the epithelial surface.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Rajendran, Dr Ranjith and Johnston, William and Brown, Dr Jason and Delaney, Mr Christopher and Ramage, Professor Gordon and Butcher, Mr John and Culshaw, Professor Shauna |
Authors: | Brown, J. L., Johnston, W., Delaney, C., Rajendran, R., Butcher, J., Khan, S., Bradshaw, D., Ramage, G., and Culshaw, S. |
College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School |
Journal Name: | Scientific Reports |
Publisher: | Nature Research |
ISSN: | 2045-2322 |
ISSN (Online): | 2045-2322 |
Published Online: | 31 October 2019 |
Copyright Holders: | Copyright © The Authors 2019 |
First Published: | First published in Scientific Reports 9:15779 |
Publisher Policy: | Reproduced under a Creative Commons license |
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