Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells

Brown, J. L. , Johnston, W., Delaney, C., Rajendran, R., Butcher, J., Khan, S., Bradshaw, D., Ramage, G. and Culshaw, S. (2019) Biofilm-stimulated epithelium modulates the inflammatory responses in co-cultured immune cells. Scientific Reports, 9, 15779. (doi:10.1038/s41598-019-52115-7) (PMID:31673005) (PMCID:PMC6823452)

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Abstract

The gingival epithelium is a physical and immunological barrier to the microbiota of the oral cavity, which interact through soluble mediators with the immune cells that patrol the tissue at the gingival epithelium. We sought to develop a three-dimensional gingivae-biofilm interface model using a commercially available gingival epithelium to study the tissue inflammatory response to oral biofilms associated with “health”, “gingivitis” and “periodontitis”. These biofilms were developed by sequential addition of microorganisms to mimic the formation of supra- and sub-gingival plaque in vivo. Secondly, to mimic the interactions between gingival epithelium and immune cells in vivo, we integrated peripheral blood mononuclear cells and CD14+ monocytes into our three-dimensional model and were able to assess the inflammatory response in the immune cells cultured with and without gingival epithelium. We describe a differential inflammatory response in immune cells cultured with epithelial tissue, and more so following incubation with epithelium stimulated by “gingivitis-associated” biofilm. These results suggest that gingival epithelium-derived soluble mediators may control the inflammatory status of immune cells in vitro, and therefore targeting of the epithelial response may offer novel therapies. This multi-cellular interface model, both of microbial and host origin, offers a robust in vitro platform to investigate host-pathogens at the epithelial surface.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Delaney, Christopher and Ramage, Professor Gordon and Butcher, Mr John and Rajendran, Dr Ranjith and Johnston, William and Brown, Mr Jason and Culshaw, Professor Shauna
Authors: Brown, J. L., Johnston, W., Delaney, C., Rajendran, R., Butcher, J., Khan, S., Bradshaw, D., Ramage, G., and Culshaw, S.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Dental School
Journal Name:Scientific Reports
Publisher:Nature Research
ISSN:2045-2322
ISSN (Online):2045-2322
Published Online:31 October 2019
Copyright Holders:Copyright © The Authors 2019
First Published:First published in Scientific Reports 9:15779
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
173112Understanding processes and mechanisms affecting the oral microbiome using OMICs approachesGordon RamageBiotechnology and Biological Sciences Research Council (BBSRC)BB/P504567/1Med - Dental School