Regulation of RKIP binding to the n-region of the Raf-1 kinase

Park, S., Rath, O., Beach, S., Xiang, X., Kelly, S. M., Luo, Z., Kolch, W. and Yeung, K. C. (2006) Regulation of RKIP binding to the n-region of the Raf-1 kinase. FEBS Letters, 580(27), pp. 6405-6412. (doi: 10.1016/j.febslet.2006.10.054)

Abstract

The Raf kinase inhibitory protein (RKIP) binds to Raf-1 interfering with binding of the MEK substrate and potentially also Raf-1 activation. In response to mitogen stimulation RKIP dissociates from Raf-1 and later re-associates. Here, using a combination of mutational approaches, biochemical studies, peptide arrays and plasmon surface resonance (BIAcore), we fine map and characterize a minimal 24 amino acid long RKIP binding domain in the Raf-1 N-region, which consists of constitutive elements at both flanks and a center element that is regulated by phosphorylation and enhances the re-binding of RKIP to Raf-1 in the later phase of mitogen stimulation.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Kolch, Prof Walter and Rath, Dr Oliver
• Authors: Park, S., Rath, O., Beach, S., Xiang, X., Kelly, S. M., Luo, Z., Kolch, W., and Yeung, K. C.
• College/School: College of Medical Veterinary and Life Sciences
• Journal Name: FEBS Letters
• Publisher: Elsevier
• ISSN: 0014-5793
• ISSN (Online): 1873-3468
• Published Online: 03 November 2006