Characterisation of the QM gene of Trypanosoma brucei

Lillico, S. G., Mottram, J. C. , Murphy, N. B. and Welburn, S. C. (2002) Characterisation of the QM gene of Trypanosoma brucei. FEMS Microbiology Letters, 211(2), pp. 123-128. (doi: 10.1111/j.1574-6968.2002.tb11213.x)

Abstract

The QM protein has been reported to have roles in both tumour suppression and transcription factor regulation in vertebrate cells, and in ribosome stability in both yeast and mammals. The present study isolated the QM gene of <i>Trypanosoma brucei</i> and determined its sequence. Alignment with QM sequences from <i>Saccharomyces cerevisiae, Arabidopsis thaliana, Drosophila melanogaster</i> and <i>Homo sapiens</i> revealed greater than 60% identity. Southern blot analysis revealed multiple copies of <i>QM</i> within the trypanosome genome. An epitope tag was inserted into the C-terminus of the <i>T. brucei</i> QM and the protein expressed under inducible control in procyclic form trypanosomes. Immune fluorescence microscopy revealed co-localisation with the GPI:protein transamidase component, GPI8, a distribution indicative of ribosome association with the rough endoplasmic reticulum.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Mottram, Professor Jeremy
• Authors: Lillico, S. G., Mottram, J. C., Murphy, N. B., and Welburn, S. C.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity
• Journal Name: FEMS Microbiology Letters
• Publisher: Wiley
• ISSN: 0378-1097
• ISSN (Online): 1574-6968
• Published Online: 09 January 2006