Molecular detection and genetic characterization of Bartonella species from rodents and their associated ectoparasites from northern Tanzania

Theonest, N. O. et al. (2019) Molecular detection and genetic characterization of Bartonella species from rodents and their associated ectoparasites from northern Tanzania. PLoS ONE, 14(10), e0223667. (doi:10.1371/journal.pone.0223667) (PMID:31613914)

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Abstract

Background: Bartonellae are intracellular bacteria, which can cause persistent bacteraemia in humans and a variety of animals. Several rodent-associated Bartonella species are human pathogens but data on their global distribution and epidemiology are limited. The aims of the study were to: 1) determine the prevalence of Bartonella infection in rodents and fleas; 2) identify risk factors for Bartonella infection in rodents; and 3) characterize the Bartonella genotypes present in these rodent and flea populations. Methods and results: Spleen samples collected from 381 rodents representing six different species were tested for the presence of Bartonella DNA, which was detected in 57 individuals (15.0%; 95% CI 11.3–18.5), of three rodent species (Rattus rattus n = 54, Mastomys natalensis n = 2 and Paraxerus flavovottis n = 1) using a qPCR targeting the ssrA gene. Considering R. rattus individuals only, risk factor analysis indicated that Bartonella infection was more likely in reproductively mature as compared to immature individuals (OR = 3.42, p <0.001). Bartonella DNA was also detected in 53 of 193 Xenopsylla cheopis fleas (27.5%: 95% CI 21.3–34.3) collected from R.rattus individuals. Analysis of ssrA and gltA sequences from rodent spleens and ssrA sequences from fleas identified multiple genotypes closely related (≥ 97% similar) to several known or suspected zoonotic Bartonella species, including B. tribocorum, B. rochalimae, B. elizabethae and B. quintana. Conclusions: The ssrA and gltA sequences obtained from rodent spleens and ssrA sequences obtained from fleas reveal the presence of a diverse set of Bartonella genotypes and increase our understanding of the bartonellae present in Tanzanian. Further studies are needed to fully characterise the prevalence, genotypes and diversity of Bartonella in different host populations and their potential impacts on human health.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Allan, Dr Kathryn and Mable, Professor Barbara and Halliday, Dr Jo and Carter, Mr Ryan and Haydon, Professor Daniel and Thomas, Dr Kate
Creator Roles:
Carter, R. W.Formal analysis, Methodology, Writing – original draft, Writing – review and editing
Mable, B. K.Investigation, Supervision, Writing – review and editing
Thomas, K. M.Conceptualization, Formal analysis, Investigation
Haydon, D. T.Conceptualization, Formal analysis, Funding acquisition, Investigation, Resources, Supervision, Writing – review and editing
Allan, K. J.Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Supervision, Writing – original draft, Writing – review and editing
Halliday, J. E.B.Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review and editing
Authors: Theonest, N. O., Carter, R. W., Amani, N., Doherty, S. L., Hugho, E., Keyyu, J. D., Mable, B. K., Shirima, G. M., Tarimo, R., Thomas, K. M., Haydon, D. T., Buza, J. J., Allan, K. J., and Halliday, J. E.B.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
College of Medical Veterinary and Life Sciences > School of Veterinary Medicine
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2019 Theonest et al.
First Published:First published in PLoS ONE 14(10): e0223667
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.859

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
170521A One-Health approach to dissecting the diverse zoonotic causes of non-malaria febrile illnessDaniel HaydonThe Royal Society (ROYSOC)AA130131Institute of Biodiversity, Animal Health and Comparative Medicine
165842Leptospirosis in Tanzania; a study of the role of rodents in an emerging public health problem.Sarah CleavelandWellcome Trust (WELLCOTR)096400/Z/11/ZInstitute of Biodiversity, Animal Health and Comparative Medicine
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190785Molecular epidemology of brucellosis in northern TanzaniaDaniel HaydonBiotechnology and Biological Sciences Research Council (BBSRC)BB/L018845/1Institute of Biodiversity, Animal Health and Comparative Medicine
190821Hazards associated with zoonotic enteric pathogens in emerging livestock meat pathways (HAZEL)Ruth ZadoksBiotechnology and Biological Sciences Research Council (BBSRC)BB/L017679/1Institute of Biodiversity, Animal Health and Comparative Medicine

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