Theonest, N. O. et al. (2019) Molecular detection and genetic characterization of Bartonella species from rodents and their associated ectoparasites from northern Tanzania. PLoS ONE, 14(10), e0223667. (doi: 10.1371/journal.pone.0223667) (PMID:31613914) (PMCID:PMC6793857)
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Abstract
Background: Bartonellae are intracellular bacteria, which can cause persistent bacteraemia in humans and a variety of animals. Several rodent-associated Bartonella species are human pathogens but data on their global distribution and epidemiology are limited. The aims of the study were to: 1) determine the prevalence of Bartonella infection in rodents and fleas; 2) identify risk factors for Bartonella infection in rodents; and 3) characterize the Bartonella genotypes present in these rodent and flea populations. Methods and results: Spleen samples collected from 381 rodents representing six different species were tested for the presence of Bartonella DNA, which was detected in 57 individuals (15.0%; 95% CI 11.3–18.5), of three rodent species (Rattus rattus n = 54, Mastomys natalensis n = 2 and Paraxerus flavovottis n = 1) using a qPCR targeting the ssrA gene. Considering R. rattus individuals only, risk factor analysis indicated that Bartonella infection was more likely in reproductively mature as compared to immature individuals (OR = 3.42, p <0.001). Bartonella DNA was also detected in 53 of 193 Xenopsylla cheopis fleas (27.5%: 95% CI 21.3–34.3) collected from R.rattus individuals. Analysis of ssrA and gltA sequences from rodent spleens and ssrA sequences from fleas identified multiple genotypes closely related (≥ 97% similar) to several known or suspected zoonotic Bartonella species, including B. tribocorum, B. rochalimae, B. elizabethae and B. quintana. Conclusions: The ssrA and gltA sequences obtained from rodent spleens and ssrA sequences obtained from fleas reveal the presence of a diverse set of Bartonella genotypes and increase our understanding of the bartonellae present in Tanzanian. Further studies are needed to fully characterise the prevalence, genotypes and diversity of Bartonella in different host populations and their potential impacts on human health.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Haydon, Professor Daniel and Halliday, Dr Jo and Allan, Dr Kathryn and Mable, Professor Barbara and Carter, Mr Ryan and Thomas, Dr Kate |
| Creator Roles: | Carter, R. W.Formal analysis, Methodology, Writing – original draft, Writing – review and editing Mable, B. K.Investigation, Supervision, Writing – review and editing Thomas, K. M.Conceptualization, Formal analysis, Investigation Haydon, D. T.Conceptualization, Formal analysis, Funding acquisition, Investigation, Resources, Supervision, Writing – review and editing Allan, K. J.Conceptualization, Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Resources, Supervision, Writing – original draft, Writing – review and editing Halliday, J. E.B.Data curation, Formal analysis, Funding acquisition, Investigation, Methodology, Supervision, Writing – original draft, Writing – review and editing |
| Authors: | Theonest, N. O., Carter, R. W., Amani, N., Doherty, S. L., Hugho, E., Keyyu, J. D., Mable, B. K., Shirima, G. M., Tarimo, R., Thomas, K. M., Haydon, D. T., Buza, J. J., Allan, K. J., and Halliday, J. E.B. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | PLoS ONE |
| Publisher: | Public Library of Science |
| ISSN: | 1932-6203 |
| ISSN (Online): | 1932-6203 |
| Copyright Holders: | Copyright © 2019 Theonest et al. |
| First Published: | First published in PLoS ONE 14(10): e0223667 |
| Publisher Policy: | Reproduced under a Creative Commons License |
| Data DOI: | 10.5525/gla.researchdata.859 |
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