Scott, P.H., Cairns, C.A., Sutcliffe, J.E., Alzuherri, H.M., McLees, A., Winter, A.G. and White, R.J. (2001) Regulation of RNA polymerase III transcription during cell cycle entry. Journal of Biological Chemistry, 276(2), pp. 1005-1014. (doi: 10.1074/jbc.M005417200)
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Abstract
Increased rates of RNA polymerase (pol) III transcription constitute a central feature of the mitogenic response, but little is known about the mechanism(s) responsible. We demonstrate that the retinoblastoma protein RB plays a major role in suppressing pol III transcription in growth-arrested fibroblasts. RB knockout cells are compromised in their ability to down-regulate pol III following serum withdrawal. RB binds and represses the pol III-specific transcription factor TFIIIB during G0 and early G1, but this interaction decreases as cells approach S phase. Full induction of pol III coincides with mid- to late G1 phase, when RB becomes phosphorylated by cyclin D- and E-dependent kinases. TFIIIB only associates with the underphosphorylated form of RB, and overexpression of cyclins D and E stimulates pol III transcription in vivo. The RB-related protein p130 also contributes to the repression of TFIIIB in growth-arrested fibroblasts. These observations provide insight into the mechanisms responsible for controlling pol III transcription during the switch between growth and quiescence.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | White, Prof Robert and Scott, Dr Pamela |
Authors: | Scott, P.H., Cairns, C.A., Sutcliffe, J.E., Alzuherri, H.M., McLees, A., Winter, A.G., and White, R.J. |
Subjects: | Q Science > QH Natural history > QH345 Biochemistry |
College/School: | College of Medical Veterinary and Life Sciences > School of Cancer Sciences |
Journal Name: | Journal of Biological Chemistry |
Journal Abbr.: | J Biol Chem. |
Publisher: | American Society for Biochemistry and Molecular Biology, Inc. |
ISSN: | 0021-9258 |
ISSN (Online): | 1083-351X |
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