Aberrant expression of TopBP1 in breast cancer

Going, J.J., Nixon, C., Dornan, E.S., Boner, W. , Donaldson, M.M. and Morgan, I.M. (2007) Aberrant expression of TopBP1 in breast cancer. Histopathology, 50(4), pp. 418-424. (doi:10.1111/j.1365-2559.2007.02622.x)

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Abstract

Aims: The TopBP1 protein includes eight BRCT domains (originally identified in BRCA1) and has homology with BRCA1 over the carboxyl terminal half of the protein. The aim of this study was to determine whether TopBP1 is aberrantly expressed in breast cancer. Methods and results: Sixty-one breast carcinomas from an unselected consecutive patient cohort were studied along with 12 samples of breast tissue from cosmetic breast reduction surgery; these were analysed immunohistochemically for TopBP1 expression using a rabbit polyclonal antibody. This antibody was validated in immunoprecipitation and immunofluorescence experiments. Immunohistochemical analysis demonstrated that TopBP1 was expressed almost exclusively in the nuclei of the normal breast epithelium. However, in a significant number of breast carcinomas TopBP1 was aberrantly expressed, as it was detected in the cytoplasm and nucleus of some tumours and exclusively in the cytoplasm of others. In two out of 61 carcinomas investigated, no TopBP1 expression was detected. Conclusions: For the first time this report demonstrates aberrant expression of the TopBP1 protein in breast carcinoma. We propose TOPBP1 as a breast cancer susceptibility gene.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Morgan, Professor Iain and Dornan, Mr Edward and Going, Dr James and Boner, Dr Winifred and Donaldson, Dr Mary
Authors: Going, J.J., Nixon, C., Dornan, E.S., Boner, W., Donaldson, M.M., and Morgan, I.M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cancer Sciences
College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing > Clinical Specialities
Journal Name:Histopathology
ISSN:0309-0167
ISSN (Online):1365-2559
Published Online:28 February 2007

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