Next-generation analysis of trypanosomatid genome stability and instability

Briggs, E. M., Marques, C. A., Reis-Cunha, J., Black, J., Campbell, S., Damasceno, J., Bartholomeu, D., Crouch, K. and McCulloch, R. (2020) Next-generation analysis of trypanosomatid genome stability and instability. In: Michels, P. A.M., Ginger, M. L. and Zilberstein, D. (eds.) Trypanosomatids: Methods and Protocols. Series: Methods in Molecular Biology (2116). Springer, pp. 225-262. ISBN 9781071602935 (doi:10.1007/978-1-0716-0294-2_15)

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Abstract

Understanding the rate and patterns of genome variation is becoming ever more amenable to whole-genome analysis through advances in DNA sequencing, which may, at least in some circumstances, have supplanted more localized analyses by cellular and genetic approaches. Whole-genome analyses can utilize both short- and long-read sequence technologies. Here we describe how sequence generated by these approaches has been used in trypanosomatids to examine DNA replication dynamics, the accumulation of modified histone H2A due to genome damage, and evaluation of genome variation, focusing on ploidy change.

Item Type:Book Sections
Status:Published
Glasgow Author(s) Enlighten ID:Damasceno, Dr Jeziel and Black, Dr Jennifer and Briggs, Miss Emma and McCulloch, Professor Richard and Crouch, Dr Kathryn and Campbell, Dr Samantha
Authors: Briggs, E. M., Marques, C. A., Reis-Cunha, J., Black, J., Campbell, S., Damasceno, J., Bartholomeu, D., Crouch, K., and McCulloch, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Publisher:Springer
ISBN:9781071602935
Copyright Holders:Copyright © The Authors
First Published:First published in Methods in Molecular Biology 2116:225-262
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
167605Kinase dependent control of DNA replication and repair as a drug target in Trypanosoma brucei.Richard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/K006495/1III - Parasitology
17115214CONFAP Understanding diverged genome repair and replication functions in trypanosomatid parasitesRichard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/M028909/1III - Parasitology
173173How do common and diverged features of the replicative stress response shape the biology of TriTrypRichard McCullochBiotechnology and Biological Sciences Research Council (BBSRC)BB/N016165/1III - Parasitology
303109Challenging trypanosome antigenic variation paradigms using natural systemsRichard McCullochWellcome Trust (WELLCOTR)4251315 (206815/Z/17/Z)III - Parasitology
170547The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/ZRIII - Parasitology