Postmortem observations on rumen wall histology and gene expression and ruminal and caecal content of beef cattle fattened on barley-based rations

Jonsson, N.N. et al. (2019) Postmortem observations on rumen wall histology and gene expression and ruminal and caecal content of beef cattle fattened on barley-based rations. Animal, 14(7), pp. 1447-1460. (doi: 10.1017/S1751731119002878) (PMID:31875798)

[img]
Preview
Text
197256.pdf - Accepted Version
Available under License Creative Commons Attribution Non-commercial No Derivatives.

584kB
[img]
Preview
Text
197256Suppl.pdf - Supplemental Material

367kB

Abstract

Sub-acute ruminal acidosis (SARA) can reduce the production efficiency and impair the welfare of cattle, potentially in all production systems. The aim of this study was to characterise measurable postmortem observations from divergently managed intensive beef finishing farms with high rates of concentrate feeding. At the time of slaughter, we obtained samples from 19 to 20 animals on each of 6 beef finishing units (119 animals in total) with diverse feeding practices, which had been subjectively classified as being high risk (three farms) or low risk (three farms) for SARA on the basis of the proportions of barley, silage and straw in the ration. We measured the concentrations of histamine, lipopolysaccharide (LPS), lactate and other short-chain fatty acids (SCFAs) in ruminal fluid, LPS and SCFA in caecal fluid. We also took samples of the ventral blind sac of the rumen for histopathology, immunohistopathology and gene expression. Subjective assessments were made of the presence of lesions on the ruminal wall, the colour of the lining of the ruminal wall and the shape of the ruminal papillae. Almost all variables differed significantly and substantially among farms. Very few pathological changes were detected in any of the rumens examined. The animals on the high-risk diets had lower concentrations of SCFA and higher concentrations of lactate and LPS in the ruminal fluid. Higher LPS concentrations were found in the caecum than the rumen but were not related to the risk status of the farm. The diameters of the stratum granulosum, stratum corneum and of the vasculature of the papillae, and the expression of the gene TLR4 in the ruminal epithelium were all increased on the high-risk farms. The expression of IFN-γ and IL-1β and the counts of cluster of differentiation 3 positive and major histocompatibility complex class two positive cells were lower on the high-risk farms. High among-farm variation and the unbalanced design inherent in this type of study in the field prevented confident assignment of variation in the dependent variables to individual dietary components; however, the CP percentage of the total mixed ration DM was the factor that was most consistently associated with the variables of interest. Despite the strong effect of farm on the measured variables, there was wide inter-animal variation.

Item Type:Articles
Additional Information:This project was supported by the Biotechnology and Biological Research Council, UK (Industrial Partnership Award BB/J016373/1, BB/J016608/1 and BB/J018120/1). The Rowett Institute of Nutrition and Health is funded by the Rural and Environment Science and Analytical Services Division of the Scottish Government.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jonsson, Professor Nicholas and Koh-Tan, Dr Caline and Ferguson, Dr Holly
Authors: Jonsson, N.N., Ferguson, H.J., Koh-Tan, H.H.C., McCartney, C.A., Cernat, R.C., Strachan, E.M., Thomson, W., Snelling, T.J., Harvey, C.D., Andonovic, I., Michie, C., and Wallace, R.J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:Animal
Publisher:Cambridge University Press
ISSN:1751-7311
ISSN (Online):2076-2615
Published Online:26 December 2019
Copyright Holders:Copyright © The Animal Consortium 2019
First Published:First published in Animal 14(7):1447-1460
Publisher Policy:Reproduced in accordance with the publisher copyright policy
Data DOI:10.5525/gla.researchdata.710

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
167846Sub-acute and acute ruminal acidosis; an interdisciplinary approach to understand and prevent a multifactorial disease.Nicholas JonssonBiotechnology and Biological Sciences Research Council (BBSRC)BB/J018120/1Institute of Biodiversity, Animal Health and Comparative Medicine