Acosta, H., Burchmore, R. , Naula, C. , Gualdrón-López, M., Quintero-Troconis, E., Cáceres, A. J., Michels, P. A.M., Concepción, J. L. and Quiñones, W. (2019) Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes. Molecular and Biochemical Parasitology, 229, pp. 62-74. (doi: 10.1016/j.molbiopara.2019.02.008) (PMID:30831156) (PMCID:PMC7082770)
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Abstract
In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmentalized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as β-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite’s glycosomes.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and Naula, Dr Christina |
| Authors: | Acosta, H., Burchmore, R., Naula, C., Gualdrón-López, M., Quintero-Troconis, E., Cáceres, A. J., Michels, P. A.M., Concepción, J. L., and Quiñones, W. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
| Journal Name: | Molecular and Biochemical Parasitology |
| Publisher: | Elsevier |
| ISSN: | 0166-6851 |
| ISSN (Online): | 1872-9428 |
| Published Online: | 01 March 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Molecular and Biochemical Parasitology 229: 62-74 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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