Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes

Acosta, H., Burchmore, R. , Naula, C., Gualdrón-López, M., Quintero-Troconis, E., Cáceres, A. J., Michels, P. A.M., Concepción, J. L. and Quiñones, W. (2019) Proteomic analysis of glycosomes from Trypanosoma cruzi epimastigotes. Molecular and Biochemical Parasitology, 229, pp. 62-74. (doi: 10.1016/j.molbiopara.2019.02.008) (PMID:30831156)

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Abstract

In Trypanosoma cruzi, the causal agent of Chagas disease, the first seven steps of glycolysis are compartmentalized in glycosomes, which are authentic but specialized peroxisomes. Besides glycolysis, activity of enzymes of other metabolic processes have been reported to be present in glycosomes, such as β-oxidation of fatty acids, purine salvage, pentose-phosphate pathway, gluconeogenesis and biosynthesis of ether-lipids, isoprenoids, sterols and pyrimidines. In this study, we have purified glycosomes from T. cruzi epimastigotes, collected the soluble and membrane fractions of these organelles, and separated peripheral and integral membrane proteins by Na2CO3 treatment and osmotic shock. Proteomic analysis was performed on each of these fractions, allowing us to confirm the presence of enzymes involved in various metabolic pathways as well as identify new components of this parasite’s glycosomes.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Naula, Dr Christina and Burchmore, Dr Richard
Authors: Acosta, H., Burchmore, R., Naula, C., Gualdrón-López, M., Quintero-Troconis, E., Cáceres, A. J., Michels, P. A.M., Concepción, J. L., and Quiñones, W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Molecular and Biochemical Parasitology
Publisher:Elsevier
ISSN:0166-6851
ISSN (Online):1872-9428
Published Online:01 March 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Molecular and Biochemical Parasitology 229: 62-74
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
190536Integrated Health - Polyomics and Systems Biomedicine (ISSF Bid)Anna DominiczakWellcome Trust (WELLCOTR)097821/Z/11/ZInstitute of Cardiovascular & Medical Sciences