Analysis of time-correlated single photon counting data: a comparative evaluation of deterministic and probabilistic approaches

Smith, D. A., McKenzie, G. , Jones, A. C. and Smith, T. A. (2017) Analysis of time-correlated single photon counting data: a comparative evaluation of deterministic and probabilistic approaches. Methods and Applications in Fluorescence, 5(4), 042001. (doi: 10.1088/2050-6120/aa8055) (PMID:29063861)

Abstract

We review various methods for analysing time-resolved fluorescence data acquired using the time-correlated single photon counting method in an attempt to evaluate their benefits and limitations. We have applied these methods to both experimental and simulated data. The relative merits of using deterministic approaches, such as the commonly used iterative reconvolution method, and probabilistic approaches, such as the smoothed exponential series method, the maximum entropy method and recently proposed basis pursuit denoising (compressed sensing) method, are outlined. In particular, we show the value of using multiple methods to arrive at the most appropriate choice of model. We show that the use of probabilistic analysis methods can indicate whether a discrete component or distribution analysis provides the better representation of the data.

• Item Type: Articles
• Additional Information: The authors gratefully acknowledge PhD studentship funding for DAS and GMcK from the Engineering and Physical Sciences Research Council Doctoral Training Account (University of Edinburgh) and the University of Melbourne.
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: McKenzie, Dr Grant
• Authors: Smith, D. A., McKenzie, G., Jones, A. C., and Smith, T. A.
• College/School: College of Medical Veterinary and Life Sciences > School of Molecular Biosciences
• Journal Name: Methods and Applications in Fluorescence
• Publisher: IOP Publishing
• ISSN: 2050-6120
• ISSN (Online): 2050-6120
• Published Online: 18 July 2017
• Copyright Holders: Copyright © 2017 IOP Publishing Ltd
• First Published: First published in Methods and Applications in Fluorescence 5(4): 042001
• Publisher Policy: Reproduced in accordance with the publisher copyright policy
• Data DOI: 10.7488/ds/2093