Multiview microscopy of single cells through microstructure-based indirect optical manipulation

Vizsnyiczai, G., Búzás, A., Aekbote, B. L. , Fekete, T., Grexa, I., Ormos, P. and Kelemen, L. (2019) Multiview microscopy of single cells through microstructure-based indirect optical manipulation. arXiv, (Unpublished)

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Fluorescent observation of cells generally suffers from the limited axial resolution due to the elongated point spread function of the microscope optics. Consequently, three-dimensional imaging results in axial resolution being several times worse than the transversal. The optical solutions to this problem usually require complicated optics and extreme spatial stability. A straightforward way to eliminate anisotropic resolution is to fuse images recorded from multiple viewing directions achieved mostly by the mechanical rotation of the entire sample. In the presented approach, multiview imaging of single cells is implemented by rotating them around an axis perpendicular to the optical axis by means of holographic optical tweezers. For this, the cells are indirectly trapped and manipulated with special microtools made with two-photon polymerization. The cell is firmly attached to the microtool and is precisely manipulated with 6 degrees of freedom. The total control over the cells position allows for its multiview fluorescence imaging from arbitrarily selected directions. The image stacks obtained this way are combined into one 3D image array with a special image processing algorithm resulting in isotropic optical resolution. The presented tool and manipulation scheme can be readily applied in various microscope platforms.

Item Type:Articles
Glasgow Author(s) Enlighten ID:Aekbote, Dr Badri
Authors: Vizsnyiczai, G., Búzás, A., Aekbote, B. L., Fekete, T., Grexa, I., Ormos, P., and Kelemen, L.
College/School:College of Science and Engineering > School of Engineering > Biomedical Engineering
Journal Name:arXiv
Copyright Holders:Copyright © 2019 The Authors
Publisher Policy:Reproduced with the permission of the Author

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