Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart

Taylor, J. M. , Nelson, C. J. , Bruton, F. A., Baghbadrani, A. K., Buckley, C., Tucker, C. S., Rossi, A. G., Mullins, J. J. and Denvir, M. A. (2019) Adaptive prospective optical gating enables day-long 3D time-lapse imaging of the beating embryonic zebrafish heart. Nature Communications, 10, 5173. (doi: 10.1038/s41467-019-13112-6) (PMID:31729395)

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Abstract

Three-dimensional fluorescence time-lapse imaging of the beating heart is extremely challenging, due to the heart’s constant motion and a need to avoid pharmacological or phototoxic damage. Although real-time triggered imaging can computationally “freeze” the heart for 3D imaging, no previous algorithm has been able to maintain phase-lock across developmental timescales. We report a new algorithm capable of maintaining day-long phase-lock, permitting routine acquisition of synchronised 3D + time video time-lapse datasets of the beating zebrafish heart. This approach has enabled us for the first time to directly observe detailed developmental and cellular processes in the beating heart, revealing the dynamics of the immune response to injury and witnessing intriguing proliferative events that challenge the established literature on cardiac trabeculation. Our approach opens up exciting new opportunities for direct time-lapse imaging studies over a 24-hour time course, to understand the cellular mechanisms underlying cardiac development, repair and regeneration.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nelson, Dr Chas and Taylor, Dr Jonathan
Authors: Taylor, J. M., Nelson, C. J., Bruton, F. A., Baghbadrani, A. K., Buckley, C., Tucker, C. S., Rossi, A. G., Mullins, J. J., and Denvir, M. A.
College/School:College of Science and Engineering > School of Physics and Astronomy
Journal Name:Nature Communications
Publisher:Nature Research
ISSN:2041-1723
ISSN (Online):2041-1723
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Nature Communications 10: 5173
Publisher Policy:Reproduced under a Creative Commons License
Data DOI:10.5525/gla.researchdata.729

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
642161Development and optimisation of synchronised 3D in-vivo imaging of the embryonic and juvenile zebrafish heartJonathan TaylorBritish Heart Foundation (BHF)NH/14/2/31074S&E P&A - PHYSICS & ASTRONOMY
692111University of Glasgow Experimental Equipment ProposalAndrew HarveyEngineering and Physical Sciences Research Council (EPSRC)EP/M028135/1S&E P&A - PHYSICS & ASTRONOMY
641351High speed flow field mapping in the embryonic zebrafish heartJonathan TaylorThe Royal Society (ROYSOC)RG130249S&E P&A - PHYSICS & ASTRONOMY
738201EPSRC DTP 16/17 and 17/18Mary Beth KneafseyEngineering and Physical Sciences Research Council (EPSRC)EP/N509668/1R&I - RESEARCH STRATEGY & INNOVATION
3022540Computational 3D imaging of highly dynamic live biological specimensJonathan TaylorThe Royal Society of Edinburgh (ROYSOCED)58915P&S - Physics & Astronomy