Hyperacetylated histones facilitate chromatin assembly in vitro

Cotten, M. and Chalkley, R. (1985) Hyperacetylated histones facilitate chromatin assembly in vitro. Nucleic Acids Research, 13(2), pp. 401-414. (doi:10.1093/nar/13.2.401) (PMID:2582355) (PMCID:PMC341004)

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Abstract

We have examined the effect of histone acetylation on the in vitro assembly of nucleosomes with DNA and purified histones at physiological ionic strength in the presence of polyglutamic acid. We have found that hyperacetylated histones assemble nucleosomes with greater efficiency, and to a greater extent, than either control or hypoacetylated histones. Assembly reactions were performed over a range of histone to DNA ratios (0.25 to 3.0, w/w) and polyglutamic acid to histone ratios (0 to 1.6, w/w). Although polyglutamic acid may act as a sink to prevent nonspecific histone-DNA interactions, our data suggest that the polyanion primarily facilitates the assembly of nucleosomes by organizing histones into a form that is amenable to deposition.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cotten, Professor Matthew
Authors: Cotten, M., and Chalkley, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nucleic Acids Research
Publisher:IRL Press
ISSN:0305-1048
ISSN (Online):1362-4962

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