Fos is an essential component of the mammalian UV response

Schreiber, M., Baumann, B., Cotten, M. , Angel, P. and Wagner, E.F. (1995) Fos is an essential component of the mammalian UV response. EMBO Journal, 14(21), pp. 5338-5349. (doi: 10.1002/j.1460-2075.1995.tb00218.x) (PMID:7489723) (PMCID:PMC394643)

Abstract

Mouse 3T3 fibroblasts lacking c‐fos were employed to demonstrate an essential function of the UV‐inducible transcription factor AP‐1 (Fos/Jun) in the response to the cytotoxic effects of short‐wavelength ultraviolet (UVC) radiation. Clonogenic survival and proliferation of cells lacking c‐fos were drastically reduced following UV irradiation. This UV hypersensitivity manifests itself primarily in increased cell death, partly by apoptosis, and prolonged recovery time from UV‐induced cell cycle arrest. Co‐culture with wild‐type cells did not ameliorate the hypersensitivity of mutant cells. Transcriptional induction of the c‐Fos target genes collagenase I, stromelysin‐1 and stromelysin‐2 by UV is almost absent in cells lacking c‐fos which correlates with a reduced UV induction of AP‐1 DNA‐binding and transactivation activity. The repair of UV‐induced DNA lesions was not affected, as shown by unscheduled DNA synthesis and host cell reactivation assays. These data demonstrate that c‐Fos is involved in a novel protective function other than DNA repair against the harmful consequences of UVC.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cotten, Professor Matthew
• Authors: Schreiber, M., Baumann, B., Cotten, M., Angel, P., and Wagner, E.F.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: EMBO Journal
• Publisher: Oxford University Press
• ISSN: 0261-4189
• ISSN (Online): 1460-2075