Transient transfection of mammary epithelial cells with a PEI/DNA/adenovirus system

Bischof, J., Vietor, I., Cotten, M. and Huber, L.A. (1999) Transient transfection of mammary epithelial cells with a PEI/DNA/adenovirus system. Biological Chemistry, 380(2), pp. 269-273. (doi: 10.1515/bc.1999.036) (PMID:10195435)

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Abstract

Studies on epithelial cells often require the transient expression of exogenous proteins in polarized epithelial cells. However, the major limitation of this approach has been the difficulty of obtaining transient gene expression in polarized epithelial cell cultures. We report here on the application of a polyethylenimine (PEI)/DNA/adenovirus system for efficient transient gene expression in mammary epithelial cells. Based on luciferase assay and FACScan analysis the PEI/DNA/adenovirus system is shown to be an effective and simple method for transfecting epithelial cells.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cotten, Professor Matthew
Authors: Bischof, J., Vietor, I., Cotten, M., and Huber, L.A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Biological Chemistry
Publisher:De Gruyter
ISSN:1431-6730
ISSN (Online):1437-4315

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