Microtubule-independent motility and nuclear targeting of adenoviruses with fluorescently labeled genomes

Glotzer, J. B., Michou, A.-I., Baker, A., Saltik, M. and Cotten, M. (2001) Microtubule-independent motility and nuclear targeting of adenoviruses with fluorescently labeled genomes. Journal of Virology, 75(5), pp. 2421-2434. (doi: 10.1128/jvi.75.5.2421-2434.2001) (PMID:11160745) (PMCID:PMC114825)

Abstract

A novel adenovirus system for analyzing the adenovirus entry pathway has been developed that contains green fluorescent protein bound to the encapsidated viral DNA (AdLite viruses). AdLite viruses enter host cells and accumulate around the nuclei and near the microtubule organizing centers (MTOC). In live cells, individual AdLite particles were observed trafficking both toward and away from the nucleus. Depolymerization of microtubules during infection prevented AdLite accumulation around the MTOC; however, it did not abolish perinuclear localization of AdLite particles. Furthermore, depolymerization of microtubules did not affect AdLite motility and did not affect gene expression from wild-type adenovirus and adenovirus-derived vectors. These data revealed that adenovirus intracellular motility and nuclear targeting can be supported by a mechanism that does not rely on the microtubule network.

• Item Type: Articles
• Status: Published
• Refereed: Yes
• Glasgow Author(s) Enlighten ID: Cotten, Professor Matthew
• Authors: Glotzer, J. B., Michou, A.-I., Baker, A., Saltik, M., and Cotten, M.
• College/School: College of Medical Veterinary and Life Sciences > School of Infection & Immunity; College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research
• Journal Name: Journal of Virology
• Publisher: American Society for Microbiology
• ISSN: 0022-538X
• ISSN (Online): 1098-5514