Gust, T. C., Diebold, S. S., Cotten, M. and Zenke, M. (2004) RNA-containing adenovirus/polyethylenimine transfer complexes effectively transduce dendritic cells and induce antigen-specific T cell responses. Journal of Gene Medicine, 6(4), pp. 464-470. (doi: 10.1002/jgm.492) (PMID:15079821)
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Abstract
Background: Dendritic cells (DCs) are the most potent antigen‐presenting cells in initiating primary immune responses. Given the unique properties of DCs, gene‐modified DCs represent a particularly attractive approach for immunotherapy of diseases such as cancer. Methods: Gene‐modified DCs were obtained by a receptor‐mediated gene delivery system using adenovirus (Ad) particles as ligand and RNA or DNA condensed by polyethylenimine (PEI). In vitro transcribed polyadenylated or non‐polyadenylated RNA was used. RNA‐transduced DCs were generated expressing chicken ovalbumin (OVA) or chimeric constructs thereof, and compared with DNA‐transduced DCs. Results: Ad/PEI transfection complexes efficiently delivered RNA into DCs. Such RNA‐transduced DCs induced OVA‐specific T cell responses more effectively than DNA‐transduced DCs. Furthermore, DCs transduced with polyadenylated RNA were more potent in stimulating CD4+ and CD8+ T cell responses than DCs transduced with non‐polyadenylated RNA and this was particularly important for CD4+ T cell responses. Conclusions: Ad/PEI/RNA transfection is an efficient means for generating RNA‐transduced DCs and for stimulating antigen‐specific T cell responses. Polyadenylation of RNA enhances CD8+ T cell responses and is essential for CD4+ T cell responses.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Cotten, Professor Matthew |
| Authors: | Gust, T. C., Diebold, S. S., Cotten, M., and Zenke, M. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research |
| Journal Name: | Journal of Gene Medicine |
| Publisher: | Wiley |
| ISSN: | 1099-498X |
| ISSN (Online): | 1521-2254 |
| Published Online: | 10 February 2004 |
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