Rapid outbreak sequencing of Ebola virus in Sierra Leone identifies transmission chains linked to sporadic cases

Arias, A. et al. (2016) Rapid outbreak sequencing of Ebola virus in Sierra Leone identifies transmission chains linked to sporadic cases. Virus Evolution, 2(1), vew016. (doi: 10.1093/ve/vew016) (PMID:28694998) (PMCID:PMC5499387)

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Abstract

To end the largest known outbreak of Ebola virus disease (EVD) in West Africa and to prevent new transmissions, rapid epidemiological tracing of cases and contacts was required. The ability to quickly identify unknown sources and chains of transmission is key to ending the EVD epidemic and of even greater importance in the context of recent reports of Ebola virus (EBOV) persistence in survivors. Phylogenetic analysis of complete EBOV genomes can provide important information on the source of any new infection. A local deep sequencing facility was established at the Mateneh Ebola Treatment Centre in central Sierra Leone. The facility included all wetlab and computational resources to rapidly process EBOV diagnostic samples into full genome sequences. We produced 554 EBOV genomes from EVD cases across Sierra Leone. These genomes provided a detailed description of EBOV evolution and facilitated phylogenetic tracking of new EVD cases. Importantly, we show that linked genomic and epidemiological data can not only support contact tracing but also identify unconventional transmission chains involving body fluids, including semen. Rapid EBOV genome sequencing, when linked to epidemiological information and a comprehensive database of virus sequences across the outbreak, provided a powerful tool for public health epidemic control efforts.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Cotten, Professor Matthew
Authors: Arias, A., Watson, S. J., Asogun, D., Tobin, E. A., Lu, J., Phan, M. V.T., Jah, U., Wadoum, R. E. G., Meredith, L., Thorne, L., Caddy, S., Tarawalie, A., Langat, P., Dudas, G., Faria, N. R., Dellicour, S., Kamara, A., Kargbo, B., Kamara, B. O., Gevao, S., Cooper, D., Newport, M., Horby, P., Dunning, J., Sahr, F., Brooks, T., Simpson, A. J.H., Groppelli, E., Liu, G., Mulakken, N., Rhodes, K., Akpablie, J., Yoti, Z., Lamunu, M., Vitto, E., Otim, P., Owilli, C., Boateng, I., Okoror, L., Omomoh, E., Oyakhilome, J., Omiunu, R., Yemisis, I., Adomeh, D., Ehikhiametalor, S., Akhilomen, P., Aire, C., Kurth, A., Cook, N., Baumann, J., Gabriel, M., Wolfel, R., Di Caro, A., Carroll, M. W., Gunther, S., Redd, J., Naidoo, D., Pybus, O. G., Rambaut, A., Kellam, P., Goodfellow, I., and Cotten, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Virus Evolution
Publisher:Oxford University Press
ISSN:2057-1577
ISSN (Online):2057-1577
Published Online:22 June 2016
Copyright Holders:Copyright © 2016 The Authors
First Published:First published in Virus Evolution 2(1):vew016
Publisher Policy:Reproduced under a Creative Commons License

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