Effects of host-derived chemokines on the motility and viability of Trypanosoma brucei

Alfituri, O. A., Ajibola, O., Brewer, J. M. , Garside, P. , Benson, R. A. , Peel, T., Morrison, L. J. and Mabbott, N. A. (2019) Effects of host-derived chemokines on the motility and viability of Trypanosoma brucei. Parasite Immunology, 41(2), e12609. (doi: 10.1111/pim.12609) (PMID:30525202) (PMCID:PMC6767366)

[img]
Preview
Text
194358.pdf - Published Version
Available under License Creative Commons Attribution.

2MB

Abstract

African trypanosomes (Trypanosoma brucei spp.) are extracellular, hemoflagellate, protozoan parasites. Mammalian infection begins when the tsetse fly vector injects trypanosomes into the skin during blood feeding. The trypanosomes then reach the draining lymph nodes before disseminating systemically. Intravital imaging of the skin post-tsetse fly bite revealed that trypanosomes were observed both extravascularly and intravascularly in the lymphatic vessels. Whether host-derived cues play a role in the attraction of the trypanosomes towards the lymphatic vessels to aid their dis-semination from the site of infection is not known. Since chemokines can mediate the attraction of leucocytes towards the lymphatics, in vitro chemotaxis assays were used to determine whether chemokines might also act as chemoattractants for tryp-anosomes. Although microarray data suggested that the chemokines CCL8, CCL19, CCL21, CCL27 and CXCL12 were highly expressed in mouse skin, they did not stimu-late the chemotaxis of T brucei. Certain chemokines also possess potent antimicrobial properties. However, none of the chemokines tested exerted any parasiticidal ef-fects on T brucei. Thus, our data suggest that host-derived chemokines do not act as chemoattractants for T brucei. Identification of the mechanisms used by trypano-somes to establish host infection will aid the development of novel approaches to block disease transmission.

Item Type:Articles
Keywords:Chemokines, chemotaxis, cytotoxicity, intravital imaging, skin, lymphatics, Trypanosoma brucei.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Garside, Professor Paul and Brewer, Professor James and Benson, Dr Robert and Morrison, Dr Liam
Authors: Alfituri, O. A., Ajibola, O., Brewer, J. M., Garside, P., Benson, R. A., Peel, T., Morrison, L. J., and Mabbott, N. A.
Subjects:Q Science > Q Science (General)
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Parasite Immunology
Publisher:Wiley
ISSN:0141-9838
ISSN (Online):1365-3024
Published Online:27 December 2018
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Parasite Immunology 41(2):e12609
Publisher Policy:Reproduced under a Creative Commons License

University Staff: Request a correction | Enlighten Editors: Update this record

Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
558483Investigation of early immune responses of trypanosome infection in mammals using intravital imaging tools.Liam MorrisonWellcome Trust (WELLCOTR)093594/Z/10/ZIII - BACTERIOLOGY
371799The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)104111/Z/14/Z & AIII - PARASITOLOGY