Modha, S. , Hughes, J. , Bianco, G., Ferguson, H. M. , Helm, B., Tong, L., Wilkie, G. S., Kohl, A. and Schnettler, E. (2019) Metaviromics reveals unknown viral diversity in the biting midge Culicoides impunctatus. Viruses, 11(9), 865. (doi: 10.3390/v11090865) (PMID:31533247) (PMCID:PMC6784199)
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Abstract
Biting midges (Culicoides species) are vectors of arboviruses and were responsible for the emergence and spread of Schmallenberg virus (SBV) in Europe in 2011 and are likely to be involved in the emergence of other arboviruses in Europe. Improved surveillance and better understanding of risks require a better understanding of the circulating viral diversity in these biting insects. In this study, we expand the sequence space of RNA viruses by identifying a number of novel RNA viruses from Culicoides impunctatus (biting midge) using a meta-transcriptomic approach. A novel metaviromic pipeline called MetaViC was developed specifically to identify novel virus sequence signatures from high throughput sequencing (HTS) datasets in the absence of a known host genome. MetaViC is a protein centric pipeline that looks for specific protein signatures in the reads and contigs generated as part of the pipeline. Several novel viruses, including an alphanodavirus with both segments, a novel relative of the Hubei sobemo-like virus 49, two rhabdo-like viruses and a chuvirus, were identified in the Scottish midge samples. The newly identified viruses were found to be phylogenetically distinct to those previous known. These findings expand our current knowledge of viral diversity in arthropods and especially in these understudied disease vectors.
| Item Type: | Articles |
|---|---|
| Status: | Published |
| Refereed: | Yes |
| Glasgow Author(s) Enlighten ID: | Tong, Dr Lily and Wilkie, Dr Gavin and Hughes, Dr Joseph and Schnettler, Dr Esther and Modha, Ms Sejal and Helm, Dr Barbara and Ferguson, Professor Heather and Kohl, Professor Alain |
| Creator Roles: | Modha, S.Conceptualization, Data curation, Formal analysis, Investigation, Methodology, Software, Visualization, Writing – original draft, Writing – review and editing Hughes, J.Conceptualization, Investigation, Methodology, Supervision, Visualization, Writing – original draft, Writing – review and editing Schnettler, E.Conceptualization, Formal analysis, Resources, Writing – review and editing Tong, L.Resources Wilkie, G. S.Resources Ferguson, H. M.Supervision, Writing – review and editing Helm, B.Supervision, Writing – review and editing Kohl, A.Writing – review and editing |
| Authors: | Modha, S., Hughes, J., Bianco, G., Ferguson, H. M., Helm, B., Tong, L., Wilkie, G. S., Kohl, A., and Schnettler, E. |
| College/School: | College of Medical Veterinary and Life Sciences > School of Infection & Immunity College of Medical Veterinary and Life Sciences > School of Infection & Immunity > Centre for Virus Research College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
| Journal Name: | Viruses |
| Publisher: | MDPI |
| ISSN: | 1999-4915 |
| ISSN (Online): | 1999-4915 |
| Published Online: | 17 September 2019 |
| Copyright Holders: | Copyright © 2019 The Authors |
| First Published: | First published in Viruses 11(9): 865 |
| Publisher Policy: | Reproduced under a Creative Commons License |
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