Arabidopsis JMJD5/JMJ30 acts independently of LUX ARRHYTHMO within the plant circadian clock to enable temperature compensation

Jones, M. A. , Morohashi, K., Grotewold, E. and Harmer, S. L. (2019) Arabidopsis JMJD5/JMJ30 acts independently of LUX ARRHYTHMO within the plant circadian clock to enable temperature compensation. Frontiers in Plant Science, 10, 57. (doi:10.3389/fpls.2019.00057) (PMID:30774641) (PMCID:PMC6367231)

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Abstract

The circadian system ensures that plants respond appropriately to environmental change by predicting regular transitions that occur during diel cycles. In order to be most useful, the circadian system needs to be compensated against daily and seasonal changes in temperature that would otherwise alter the pace of this biological oscillator. We demonstrate that an evening-phased protein, the putative histone demethylase JMJD5, contributes to temperature compensation. JMJD5 is co-expressed with components of the Evening Complex, an agglomeration of proteins including EARLY FLOWERING3 (ELF3), ELF4, and LUX ARRHYTHYMO (LUX), which also integrates temperature changes into the molecular clockwork. One role of the Evening Complex is to regulate expression of PSEUDORESPONSE REGULATOR9 (PRR9) and PRR7, important components of the temperature compensation mechanism. Surprisingly we find that LUX, but not other Evening Complex components, is dispensable for clock function at low temperatures. Further genetic analysis suggests JMJD5 acts in a parallel pathway to LUX within the circadian system. Although an intact JMJD5 catalytic domain is required for its function within the clock, our findings suggest JMJD5 does not directly regulate H3K36 methylation at circadian loci. Such data refine our understanding of how JMDJ5 acts within the Arabidopsis circadian system.

Item Type:Articles
Additional Information:This study was supported by the Leverhulme Trust (ECF-2012– 358 to MJ), the National Institutes of Health (R01 GM069418 to SH), the United States Department of Agriculture–National Institute of Food and Agriculture (Grant CA-D-PLB-2259-H to SH), and the National Science Foundation (Grant MCB-0418891 to EG).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Jones, Dr Matt
Authors: Jones, M. A., Morohashi, K., Grotewold, E., and Harmer, S. L.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Frontiers in Plant Science
Publisher:Frontiers Media
ISSN:1664-462X
ISSN (Online):1664-462X
Copyright Holders:Copyright © 2019 Jones, Morohashi, Grotewold and Harmer
First Published:First published in Frontiers in Plant Science 10: 57
Publisher Policy:Reproduced under a Creative Commons License

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