Mutapi, F., Burchmore, R., Mduluza, T., Foucher, A., Harcus, Y., Nicoll, G., Midzi, N., Turner, C. and Maizels, R. (2005) Praziquantel treatment of individuals exposed to Schistosoma haematobium enhances serological recognition of defined parasite antigens. Journal of Infectious Diseases, 192(6), pp. 1108-1118. (doi: 10.1086/432553) (PMID:16107967)
Full text not currently available from Enlighten.
Abstract
BackgroundSchistosomiasis is a major parasitic disease affecting >200 million people in the developing world, and 400 million people are at risk for infection. This study aimed to identify and compare proteins recognized by serum samples from schistosome-exposed individuals before and after curative praziquantel treatment MethodsProteins recognized by pooled serum samples from Schistosoma haematobium–exposed Zimbabweans were determined by 2-dimensional Western blotting and identified by mass spectrometry ResultsSerum samples recognized 71 spots, which resolved to 26 different characterized proteins. Eleven of these proteins have not previously been shown to be immunogenic in natural human infection or in experimental models of schistosomiasis, making them novel antigens in the parasite. Pretreatment serum samples recognized 59 spots, which resolved to 21 different identified proteins. Posttreatment serum samples recognized an additional 12 spots, which resolved to 8 different identified proteins. Of these 8 proteins, 3 had putative isoforms recognized before treatment, and 5 (calreticulin, tropomyosin 1, tropomyosin 2, paramyosin, and triose phosphate isomerase) did not ConclusionsThis study is the most comprehensive characterization of S. haematobium antigens to date and describes novel antigens in all schistosome species. Posttreatment results are consistent with praziquantel treatment inducing quantitative and qualitative changes in schistosome-specific antibody responses.
Item Type: | Articles |
---|---|
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Burchmore, Dr Richard and Turner, Professor Charles |
Authors: | Mutapi, F., Burchmore, R., Mduluza, T., Foucher, A., Harcus, Y., Nicoll, G., Midzi, N., Turner, C., and Maizels, R. |
College/School: | College of Medical Veterinary and Life Sciences College of Medical Veterinary and Life Sciences > School of Infection & Immunity |
Journal Name: | Journal of Infectious Diseases |
Publisher: | Oxford University Press (OUP) on behalf of Infectious Diseases Society of America |
ISSN: | 0022-1899 |
ISSN (Online): | 1537-6613 |
University Staff: Request a correction | Enlighten Editors: Update this record