A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles

Periz, J., Del Rosario, M., McStea, A., Gras, S., Loney, C., Wang, L., Martin-Fernandez, M. L. and Meissner, M. (2019) A highly dynamic F-actin network regulates transport and recycling of micronemes in Toxoplasma gondii vacuoles. Nature Communications, 10, 4183. (doi:10.1038/s41467-019-12136-2) (PMID:31519913) (PMCID:PMC6744512)

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Abstract

The obligate intracellular parasite Toxoplasma gondii replicates in an unusual process, described as internal budding. Multiple dausghter parasites are formed sequentially within a single mother cell, requiring replication and distribution of essential organelles such as micronemes. These organelles are thought to be formed de novo in the developing daughter cells. Using dual labelling of a microneme protein MIC2 and super-resolution microscopy, we show that micronemes are recycled from the mother to the forming daughter parasites using a highly dynamic F-actin network. While this recycling pathway is F-actin dependent, de novo synthesis of micronemes appears to be F-actin independent. The F-actin network connects individual parasites, supports long, multidirectional vesicular transport, and regulates transport, density and localisation of micronemal vesicles. The residual body acts as a storage and sorting station for these organelles. Our data describe an F-actin dependent mechanism in apicomplexans for transport and recycling of maternal organelles during intracellular development.

Item Type:Articles
Additional Information:J.P. was supported with a Central Laser Facility Octopus facility access award (STFC, Rutherford Appleton laboratory).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Meissner, Professor Markus and Del Rosario Minina, Mr Mario and Loney, Mr Colin and Periz, Dr Javier and Gras, Dr Simon
Authors: Periz, J., Del Rosario, M., McStea, A., Gras, S., Loney, C., Wang, L., Martin-Fernandez, M. L., and Meissner, M.
College/School:College of Medical Veterinary and Life Sciences > Institute of Infection Immunity and Inflammation
Journal Name:Nature Communications
Publisher:Nature Publishing Group
ISSN:2041-1723
ISSN (Online):2041-1723
Published Online:13 September 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Nature Communications 10:4183
Publisher Policy:Reproduced under a Creative Commons license

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
501602Systematic analysis of essential parasite genes linked to invasion of the host cell in Toxoplasma gondiiMarkus MeissnerWellcome Trust (WELLCOTR)087582/Z/08/ZIII - PARASITOLOGY
592941EndoTox: Endosomal dependent transport to the unique secretory organelles of apicomplexan parasitesMarkus MeissnerEuropean Research Council (ERC)309255III - PARASITOLOGY
371798The Wellcome Centre for Molecular Parasitology ( Core Support )Andrew WatersWellcome Trust (WELLCOTR)085349/B/08/ZIII - PARASITOLOGY

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