Antier, D., Carswell, H., Brosnan, M., Hamilton, C., Macrae, I., Groves, S., Jardine, E., Reid, J. and Dominiczak, A. (2004) Increased levels of superoxide in brains from old female rats. Free Radical Research, 38(2), pp. 177-183. (doi: 10.1080/10715760310001643294)
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Abstract
Hypertension, aging and a range of neurodegenerative diseases are associated with increased oxidative damage. The present study examined whether superoxide (O2•-) levels in brain are increased during aging in female rats, and the role of superoxide dismutase (SOD) and oestrogen in regulating O2•- levels. Young adult (3 month) and old (11 month) female spontaneously hypertensive stroke prone rats (SHRSP) and normotensive Wistar-Kyoto rats (WKY) were studied. O2•- levels were measured in brain homogenates by lucigenin chemiluminescence and SOD expression by Western blotting. Ageing significantly increased brain O2•- levels in WKY (cortex +216%, hippocampus +320%, striatum +225%) and to a greater extent in SHRSP (cortex +540%, hippocampus +580%, striatum +533%). Older SHRSP showed a decline in cortical Cu/Zn SOD expression compared to young adult SHRSP. Oestrogen did not attenuate O2•- levels. The results show a significant age-dependent increase in brain O2•- levels which is exaggerated in SHRSP. The excess cortical O2•- levels in the SHRSP may be associated with a down-regulation of Cu/Zn SOD but are not related to a decrease in oestrogen.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Hamilton, Dr Carlene and Dominiczak, Professor Anna and Macrae, Professor Mhairi and Reid, Professor John |
Authors: | Antier, D., Carswell, H., Brosnan, M., Hamilton, C., Macrae, I., Groves, S., Jardine, E., Reid, J., and Dominiczak, A. |
College/School: | College of Medical Veterinary and Life Sciences > School of Cardiovascular & Metabolic Health College of Medical Veterinary and Life Sciences > School of Psychology & Neuroscience |
Journal Name: | Free Radical Research |
ISSN: | 1071-5762 |
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