Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry

Denver, N., Khan, S., Stasinopoulos, I., Church, C., Homer, N. Z. M., Maclean, M. R. and Andrew, R. (2019) Data for analysis of catechol estrogen metabolites in human plasma by liquid chromatography tandem mass spectrometry. Data In Brief, 23, 103740. (doi: 10.1016/j.dib.2019.103740) (PMID:31372406) (PMCID:PMC6660464)

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Abstract

Analysis of catechol estrogens (2 & 4 hydroxy-estrone and estradiol) has proven troublesome by liquid chromatography tandem mass spectrometry due to their low concentrations, short half-lives and temperature-labile nature. Derivatization to methyl piperazine analogues has been reported for a panel of 9 estrogens in, "Derivatization enhances analysis of estrogens and their bioactive metabolites in human plasma by liquid chromatography tandem mass spectrometry" (Denver et al., 2019). Data show alteration of the base catalyst in this method was required to allow detection of catechol estrogens to low levels. Data also highlight the challenges faced in chromatographic separation of isomers and isotopologues, which were partially overcome by employing an extended column length and reduced oven temperature. In addition, data analysis displayed significant matrix effects during quantitation in plasma, following solid-phase extraction, despite efficient recoveries.

Item Type:Articles
Additional Information:We would like to thank the BBSRC iCASE award (BB/N503691/1) for PhD funding and to the Graham Wilson Travel Scholarship for their award to fund commuting costs for two years of the project. We would also like to thank the Wellcome Trust (202794/Z/16/Z), NC3Rs (NC/L00108X/1) and The BHF (PG/15/63/31659 and RG/16/2/32153) for funding.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Maclean, Miss Margaret and Denver, Nina
Authors: Denver, N., Khan, S., Stasinopoulos, I., Church, C., Homer, N. Z. M., Maclean, M. R., and Andrew, R.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
Journal Name:Data In Brief
Publisher:Elsevier
ISSN:2352-3409
ISSN (Online):2352-3409
Published Online:08 March 2019
Copyright Holders:Copyright © 2019 The Authors
First Published:First published in Data in Brief 23:103740
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172240Investigating oestrogen metabolism in pulmonary artery smooth muscle cellsMargaret MacLeanBritish Heart Foundation (BHF)PG/15/63/31659Institute of Cardiovascular & Medical Sciences
173026Sex and the development of pulmonary arterial hypertensionMargaret MacLeanBritish Heart Foundation (BHF)RG/16/2/32153Institute of Cardiovascular & Medical Sciences