Sadler, J. B.A., Roccisana, J., Virolainen, M., Bryant, N. J. and Gould, G. W. (2015) mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. Communicative and Integrative Biology, 8(3), e1026494. (doi: 10.1080/19420889.2015.1026494) (PMID:26479872) (PMCID:PMC4594494)
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Abstract
Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.
Item Type: | Articles |
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Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Sadler, Miss Jessica and Gould, Professor Gwyn and Roccisana, Dr Jennifer and Bryant, Dr Nia |
Authors: | Sadler, J. B.A., Roccisana, J., Virolainen, M., Bryant, N. J., and Gould, G. W. |
College/School: | College of Medical Veterinary and Life Sciences > School of Molecular Biosciences College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing |
Journal Name: | Communicative and Integrative Biology |
Publisher: | Taylor and Francis |
ISSN: | 1942-0889 |
ISSN (Online): | 1942-0889 |
Published Online: | 24 June 2015 |
Copyright Holders: | Copyright © 2014 Jessica B A Sadler, Jennifer Roccisana, Minttu Virolainen, Nia J Bryant, and Gwyn W Gould |
First Published: | First published in Communicative and Integrative Biology 8(3):e1026494 |
Publisher Policy: | Reproduced under a Creative Commons License |
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