mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes

Sadler, J. B.A., Roccisana, J., Virolainen, M., Bryant, N. J. and Gould, G. W. (2015) mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes. Communicative and Integrative Biology, 8(3), e1026494. (doi:10.1080/19420889.2015.1026494) (PMID:26479872) (PMCID:PMC4594494)

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Abstract

Insulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Depletion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose transport and GLUT4 translocation. Here we show that depletion of mVps45 selectively reduced expression of VAMPs 2 and 4, but not other VAMP isoforms. Although we did not observe direct interaction of mVps45 with any VAMP isoform; we found that the cognate binding partner of mVps45, Syntaxin 16 associates with VAMPs 2, 4, 7 and 8 in vitro. Co-immunoprecipitation experiments in 3T3-L1 adipocytes revealed an interaction between Syntaxin 16 and only VAMP4. We suggest GLUT4 trafficking is controlled by the coordinated expression of mVps45/Syntaxin 16/VAMP4, and that depletion of mVps45 regulates VAMP2 levels indirectly, perhaps via reduced trafficking into specialized subcellular compartments.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Sadler, Miss Jessica and Gould, Professor Gwyn and Roccisana, Dr Jennifer and Bryant, Dr Nia
Authors: Sadler, J. B.A., Roccisana, J., Virolainen, M., Bryant, N. J., and Gould, G. W.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Medicine, Dentistry & Nursing
Journal Name:Communicative and Integrative Biology
Publisher:Taylor and Francis
ISSN:1942-0889
ISSN (Online):1942-0889
Published Online:24 June 2015
Copyright Holders:Copyright © 2014 Jessica B A Sadler, Jennifer Roccisana, Minttu Virolainen, Nia J Bryant, and Gwyn W Gould
First Published:First published in Communicative and Integrative Biology 8(3):e1026494
Publisher Policy:Reproduced under a Creative Commons License

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