Circannual and circadian rhythms of hypothalamic DNA methyltransferase and histone deacetylase expression in male Siberian hamsters (Phodopus sungorus)

Stevenson, T. J. (2017) Circannual and circadian rhythms of hypothalamic DNA methyltransferase and histone deacetylase expression in male Siberian hamsters (Phodopus sungorus). General and Comparative Endocrinology, 243, pp. 130-137. (doi: 10.1016/j.ygcen.2016.11.011) (PMID:27916575)

[img]
Preview
Text
190944.pdf - Accepted Version

4MB

Abstract

Precise timing of gene transcription is a fundamental component of many biological rhythms. DNA methylation and histone acetylation are two epigenetic modifications that can affect the probability of gene transcription and RNA expression. Enzymes involved in DNA methylation (dnmts) have been shown to exhibit photoperiodic rhythms in expression in the hypothalamus, which coincide with hypothalamic expression of deiodinase type III (dio3), a gene involved in the photoperiodic regulation of reproduction. It is currently unknown whether enzymes involved in histone deacetylation (hdacs) also vary in response to photoperiod, nor have seasonal changes in the circadian waveforms of methylation and/or acetylation enzymes been examined. The present work documents circadian and photoperiodic changes in dnmts and hdacs in whole hypothalamic dissections obtained from male Siberian hamsters (Phodopus sungorus) after 5–6 weeks of exposure to SD. The data indicate that short days (SD) markedly inhibit dnmt3a expression, and that SD inhibition of dnmt3a was evident regardless of the alignment of circadian waveforms. Among hdacs, photoperiodic and circadian changes in expression were only observed in hdac4 expression. Recurrent temporal waveforms in epigenetic enzyme expression may provide molecular inputs to the timing systems that reprogram RNA expression to generate daily and annual phenotypic plasticity.

Item Type:Articles
Additional Information:TJS was funded by a College of Life Sciences and Medicine start-up award from the University of Aberdeen.
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Stevenson, Dr Tyler
Authors: Stevenson, T. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:General and Comparative Endocrinology
Publisher:Elsevier BV
ISSN:0016-6480
ISSN (Online):0016-6480
Published Online:01 December 2016
Copyright Holders:Copyright © 2016 Elsevier
First Published:First published in General and Comparative Endocrinology 243:130-137
Publisher Policy:Reproduced in accordance with the copyright policy of the publisher

University Staff: Request a correction | Enlighten Editors: Update this record