Rebourcet, D., Monteiro, A., Cruickshanks, L., Jeffery, N., Smith, S., Milne, L., O'Shaughnessy, P. J. and Smith, L. B. (2019) Relationship of transcriptional markers to Leydig cell number in the mouse testis. PLoS ONE, 14(7), e0219524. (doi: 10.1371/journal.pone.0219524) (PMID:31291327) (PMCID:PMC6619764)
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Abstract
Objectives: The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size. Results: Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.
Item Type: | Articles |
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Additional Information: | This work was funded by Biotechnology and Biological Sciences Research Council (BBSRC) Project grant (BB/J015105/1) (to LBS and PJO), and Medical Research Council (MRC) Programme Grant (G1100354/1 and MR/N002970/1) (to LBS). |
Status: | Published |
Refereed: | Yes |
Glasgow Author(s) Enlighten ID: | Monteiro, Dr Ana and O'Shaughnessy, Professor Peter |
Creator Roles: | Monteiro, A.Investigation, Validation O'Shaughnessy, P. J.Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Visualization, Writing – original draft, Writing – review and editing |
Authors: | Rebourcet, D., Monteiro, A., Cruickshanks, L., Jeffery, N., Smith, S., Milne, L., O'Shaughnessy, P. J., and Smith, L. B. |
College/School: | College of Medical Veterinary and Life Sciences > School of Biodiversity, One Health & Veterinary Medicine |
Journal Name: | PLoS ONE |
Publisher: | Public Library of Science |
ISSN: | 1932-6203 |
ISSN (Online): | 1932-6203 |
Copyright Holders: | Copyright © 2019 Rebourcet et al. |
First Published: | First published in PLoS ONE 14(7): e0219524 |
Publisher Policy: | Reproduced under a Creative Commons License |
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