Relationship of transcriptional markers to Leydig cell number in the mouse testis

Rebourcet, D., Monteiro, A., Cruickshanks, L., Jeffery, N., Smith, S., Milne, L., O'Shaughnessy, P. J. and Smith, L. B. (2019) Relationship of transcriptional markers to Leydig cell number in the mouse testis. PLoS ONE, 14(7), e0219524. (doi: 10.1371/journal.pone.0219524) (PMID:31291327) (PMCID:PMC6619764)

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Abstract

Objectives: The current study aims to identify markers that would reflect the number of Leydig cells present in the testis, to help determine whether labour-intensive methods such as stereology are necessary. We used our well-characterised Sertoli cell ablation model in which we have empirically established the size of the Leydig cell population, to try to identify transcriptional biomarkers indicative of population size. Results: Following characterisation of the Leydig cell population after Sertoli cell ablation in neonatal life or adulthood, we identified Hsd3b1 transcript levels as a potential indicator of Leydig cell number with utility for informing decision-making on whether to engage in time-consuming stereological cell counting analysis.

Item Type:Articles
Additional Information:This work was funded by Biotechnology and Biological Sciences Research Council (BBSRC) Project grant (BB/J015105/1) (to LBS and PJO), and Medical Research Council (MRC) Programme Grant (G1100354/1 and MR/N002970/1) (to LBS).
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:O'Shaughnessy, Professor Peter and Monteiro, Dr Ana
Creator Roles:
Monteiro, A.Investigation, Validation
O'Shaughnessy, P. J.Conceptualization, Formal analysis, Funding acquisition, Investigation, Project administration, Supervision, Visualization, Writing – original draft, Writing – review and editing
Authors: Rebourcet, D., Monteiro, A., Cruickshanks, L., Jeffery, N., Smith, S., Milne, L., O'Shaughnessy, P. J., and Smith, L. B.
College/School:College of Medical Veterinary and Life Sciences > Institute of Biodiversity Animal Health and Comparative Medicine
Journal Name:PLoS ONE
Publisher:Public Library of Science
ISSN:1932-6203
ISSN (Online):1932-6203
Copyright Holders:Copyright © 2019 Rebourcet et al.
First Published:First published in PLoS ONE 14(7): e0219524
Publisher Policy:Reproduced under a Creative Commons License

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Project CodeAward NoProject NamePrincipal InvestigatorFunder's NameFunder RefLead Dept
172424Androgens: unlocking the key drivers of male health and wellbeing RA2523Colin SelmanMedical Research Council (MRC)MR/N002970/1Institute of Biodiversity, Animal Health and Comparative Medicine