Genetic and cytological methods to study ESCRT cell cycle function in fission yeast

Rezig, I. M., Bremner, S. K., Bhutta, M. S., Salt, I. P. , Gould, G. W. and McInerny, C. J. (2019) Genetic and cytological methods to study ESCRT cell cycle function in fission yeast. In: Culetto, E. and Legouis, R. (eds.) The ESCRT Complexes: Methods and Protocols. Series: Methods in molecular biology (1998). Humana Press: New York, pp. 239-250. ISBN 9781493994915 (doi:10.1007/978-1-4939-9492-2_18)

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Abstract

The fission yeast Schizosaccharomyces pombe, an ascomycete fungus, is an established model organism for studying eukaryotic molecular and cellular events such as the cell cycle due to its powerful genetics, a sequenced genome, and the ease of molecular manipulation (Wood et al., Nature 415:871-880, 2002; Hoffman et al., Genetics 201:403-423, 2015). This chapter describes genetic and cytological methods to study endosomal sorting complex required for transport (ESCRT) function during the cell cycle in fission yeast. These include tetrad analysis to allow the creation of double mutants to test for genetic interactions by synthetic phenotype characterization, such as cellular growth and the analysis of division septa by calcofluor-white staining.

Item Type:Book Sections
Keywords:Anillin, calcofluor-white, ESCRT, fission yeast, genetic interaction, polo kinase, Schizosaccharomyces pombe, septation, synthetic phenotype, tetrad analysis.
Status:Published
Glasgow Author(s) Enlighten ID:Gould, Professor Gwyn and McInerny, Dr Chris and Salt, Dr Ian and rezig, Imane Nour El Houda and BREMNER, SHAUN
Authors: Rezig, I. M., Bremner, S. K., Bhutta, M. S., Salt, I. P., Gould, G. W., and McInerny, C. J.
College/School:College of Medical Veterinary and Life Sciences > Institute of Cardiovascular and Medical Sciences
College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
Journal Name:Methods in molecular biology (Clifton, N.J.)
Publisher:Humana Press
ISSN:1940-6029
ISBN:9781493994915
Published Online:28 June 2019

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