A general method for the localization of enzymes that produce phosphate, pyrophosphate, or CO2 after polyacrylamide gel electrophoresis

Nimmo, H.G. and Nimmo, G.A. (1982) A general method for the localization of enzymes that produce phosphate, pyrophosphate, or CO2 after polyacrylamide gel electrophoresis. Analytical Biochemistry, 121(1), pp. 17-22. (doi: 10.1016/0003-2697(82)90551-6) (PMID:6178319)

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Abstract

Previous workers have stained gels for enzymes that produce inorganic phosphate by using the insolubility of calcium phosphate. This method can also be applied to enzymes that produce pyrophosphate or CO2. The white bands of the precipitated calcium salt are clearly visible when viewed against a dark background and can be photographed or scanned. The method can be used at pH 6 and above; the level of Ca2+ required is reduced at higher pH values. The sensitivity of the method is tested by injecting the various anions into presoaked gels; as little as 10 nmol of phosphate or pyrophosphate and 100 nmol of CO2 produce clearly visible precipitates.

Item Type:Articles
Status:Published
Refereed:Yes
Glasgow Author(s) Enlighten ID:Nimmo, Dr Gillian and Nimmo, Professor Hugh
Authors: Nimmo, H.G., and Nimmo, G.A.
College/School:College of Medical Veterinary and Life Sciences > Institute of Molecular Cell and Systems Biology
College of Medical Veterinary and Life Sciences > School of Life Sciences
Journal Name:Analytical Biochemistry
Publisher:Elsevier
ISSN:0003-2697
ISSN (Online):1096-0309

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